Shoichi Sawaguchi

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Aquaporin (AQP) 5 gene was recently isolated from salivary gland and identified as a member of the AQP family. The mRNA expression and localization have been examined in several organs. The present study was focused on elucidation of AQP5 expression and localization in the eye, salivary gland, and lung in rat. RNase protection assay confirmed intense(More)
PURPOSE To determine the prevalence and risk factors for pterygium in a Japanese population aged 40 years or older on Kumejima Island, Japan. DESIGN Cross-sectional, population-based study. METHODS All residents of Kumejima Island, Japan, located in Southwestern Japan (Eastern longitude 126 degrees, 48 feet and Northern latitude 26 degrees, 20 feet),(More)
OBJECTIVE To identify molecular mechanisms of retinal responses to intraocular pressure elevation in primate experimental glaucoma. METHODS An experimental glaucoma model was created by repeated laser trabeculophotocoagulation. After the preparation of complementary DNAs from extracted total RNAs in the retinas, polymerase chain reaction (PCR) experiments(More)
PURPOSE Keratoconus is characterized by thinning and scarring of the central region of the cornea. Previous research showed that, in corneas obtained from patients with keratoconus, lysosomal enzyme activities are elevated, whereas levels of protease inhibitors such as alpha1-proteinase inhibitor are reduced. This study was undertaken to examine further the(More)
PURPOSE We attempted to analyze the three-dimensional ultrastructure of human corneal and scleral collagen fibrils with an atomic force microscope (AFM). METHODS A normal eye removed from a 66-year-old male patient during therapy was used in this study. Suspended corneal and scleral collagen fibrils were individually attached to glass slides by(More)
PURPOSE To quantitatively characterize the ultrasound biomicroscopy (UBM) configurations of the peripheral anterior chamber (AC) in primary angle closure (PAC) and its suspects and to evaluate the diagnostic performance of the UBM parameters to differentiate PAC/PAC suspects in a population-based setting. DESIGN Cross-sectional study. METHODS A random(More)
A simple and reproducible rodent glaucoma model is required to elucidate the pathophysiology of damage to the optic nerve. We developed chronically elevated intraocular pressure (IOP) unilaterally in rats by injecting india ink into the anterior chamber of one eye using a 30-gauge needle. One week later, trapped carbon particles in the chamber angle formed(More)
The present study was designed to analyze the subfibrillar structure of corneal and scleral collagen fibrils by scanning electron microscopy (SEM) and atomic force microscopy (AFM). Isolated collagen fibrils of the bovine cornea and sclera were fixed with 1% OsO4, stained with phosphotungstic acid and uranyl acetate, dehydrated in ethanol, critical(More)
This investigation examined many parameters during the course of early development of naphthalene-induced cataract in a time span of 0 to 79 days of treatment. Feeding naphthalene daily to Black-Hooded rats resulted in gradual progressive development of cataract. The first faint opacities were detectable after 7 days. Free soluble total glutathione(More)
PURPOSE To determine the role in the eye of chondromodulin (ChM)-I, which has been identified in cartilage as an angiogenic inhibitor, the expression and localization and a possible function of ChM-I were investigated. METHODS Expression and localization of ChM-I in rat eyes were examined by RNase protection assay and in situ hybridization and by(More)