Shohei Oshima

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Extracellular concentration of nitrite (NO2-), an oxidized product of nitric oxide (NO), was measured consecutively in the dorsal region of the rat suprachiasmatic nucleus (SCN) by means of in vivo microdialysis. The NO2- concentrations in the dialysates showed robust circadian rhythm under a 12:12 h light/dark cycle and were higher during the dark phase(More)
We performed x-ray crystallographic analyses of the 6-aminohexanoate oligomer hydrolase (NylC) from Agromyces sp. at 2.0 Å-resolution. This enzyme is a member of the N-terminal nucleophile hydrolase superfamily that is responsible for the degradation of the nylon-6 industry byproduct. We observed four identical heterodimers (27 kDa + 9 kDa), which resulted(More)
Oro-facial sensorimotor function conducted by the brainstem is vital to newborn mammals, and N-methyl-D-aspartate (NMDA) receptors play an important role in the regulation. Here we examined the expression of NMDA receptor subunits in the mouse hypoglossal nucleus from embryonic day 13 (E13) through postnatal day 21 (P21). Compared with other brainstem(More)
The high-affinity choline transporter CHT1 works for choline uptake in the presynaptic terminals of cholinergic neurons. We examined its expression in the hypoglossal nucleus after unilateral hypoglossal nerve transection in mice by fluorescent in situ hybridization. One week after axotomy, CHT1 mRNA expression was lost in all hypoglossal motoneurons in the(More)
Production of nitric oxide (NO) in the hypothalamic paraventricular nucleus (PVN) was examined by microdialysis in rats subjected to immobilization (IMO) stress. A dialysis probe was implanted in the posterior magnocellular subdivision of the PVN and nitrite (NO(2)(-)), an oxidized product of NO, was measured continuously. NO(2)(-) concentration in(More)
We examined the expression profile of catechol O-methyltransferase (COMT) mRNA and its protein in the neonatal rat hypothalamus by in situ hybridization and immunohistochemistry to clarify the sites of dopamine degradation. Strong COMT mRNA expression was observed in the suprachiasmatic nucleus (SCN) throughout its rostrocaudal extent at postnatal day 1(More)
Semipermeable organic-inorganic hybrid vesicles coated with a siloxane surface were spontaneously formed by simple dispersion of an organoalkoxysilane lipid in water. The hybrid vesicles allow the permeation of hydrophilic small molecules across the membrane without an introduction of a pore-forming protein.
Calcineurin is a Ca(2+) /calmodulin-dependent protein phosphatase consisting of two subunits - catalytic subunit A (CnA) and regulatory subunit B (CnB) - and plays a critical role in transducing Ca(2+) signals into cellular responses. In this study, we investigated the expression of calcineurin in the mouse developing tooth. In-situ hybridization detected(More)
In secondary ion mass spectrometry (SIMS) of organic substances, the dissociation of the sample molecules is crucial. We have developed SIMS equipment capable of bombardment, where the primary ions are argon cluster ions with kinetic energy per atom controllable down to 1  eV. We previously reported the detection of intact ions of insulin and cytochrome C(More)
From the Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, Hyogo 671-2280; Department of Life Science, Graduate School of Life Science, University of Hyogo, Hyogo 678-1297; RIKEN Harima Institute, SPring-8 Center, Hyogo 679-5148; Institute for Protein Research, Osaka University, Osaka 565-0871, Department of(More)
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