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Effect of Chemical Chaperones in Improving the Solubility of Recombinant Proteins in Escherichia coli †
- Shivcharan Prasad, Prashant B. Khadatare, I. Roy
- Medicine, Biology
- Applied and Environmental Microbiology
- 6 May 2011
ABSTRACT The recovery of active proteins from inclusion bodies usually involves chaotrope-induced denaturation, followed by refolding of the unfolded protein. The efficiency of renaturation is low,… Expand
Effect of osmolytes on the fibrillation of HypF-N.
We have studied the effect of a series of stabilizing and destabilizing osmolytes on the fibrillation pattern of a model amyloidogenic protein, HypF-N. Under mildly denaturing conditions, HypF-N… Expand
Converting Enzymes into Tools of Industrial Importance.
BACKGROUND Enzymes have applications in numerous biotechnological products and processes that are commonly used in the production of food and beverages, cleaning supplies, clothing, paper products,… Expand
Effect of disaccharides on the stabilization of bovine trypsin against detergent and autolysis
Osmolytes have been reported to stabilize biomolecules and even whole organisms against exposure to adverse environmental conditions. In this work, we report for the first time the use of some of… Expand
Design, synthesis and biological evaluation of 5-benzylidene-2-iminothiazolidin-4-ones as selective GSK-3β inhibitors.
- Minhajul Arfeen, S. Bhagat, +4 authors P. V. Bharatam
- Medicine, Chemistry
- European journal of medicinal chemistry
- 4 October 2016
In this work, iminothiazolidin-4-one derivatives were explored as selective GSK-3β inhibitors. Molecular docking analysis was carried to design a series of compounds, which were synthesized using… Expand
Length of polyglutamine tract affects secondary and tertiary structures of huntingtin protein.
- Shivang N Vachharajani, R. K. Chaudhary, Shivcharan Prasad, I. Roy
- Chemistry, Medicine
- International journal of biological…
- 1 December 2012
The role of polyglutamine (polyQ) tract on protein stability and disease pathology remains ambiguous. We monitored the unfolding/refolding patterns of huntingtin proteins with varying polyQ lengths.… Expand
Differential effect of a chemical denaturant on activity and stability of a serine protease in nonaqueous media
Abstract With reference to industrial application, reusability of the biocatalyst is an important criterion which determines the cost of the final product. Urea-induced structural perturbation of… Expand
Correlation between Al(3+) -induced thermal stability and inhibition of fibrillation of N-terminal domain of the hydrogenase maturation factor.
- Nimesh M. Sutariya, Shivcharan Prasad, D. Athavale, Rupen D. Bhavsar, I. Roy
- Chemistry, Medicine
- 1 November 2013
Fibrillation can be induced in proteins by altering solvent conditions. Stabilization of the protofibrillar structure arrests formation of longer fibers. Thermal stability and fibrillation of… Expand
A Sensitive, Rapid, and Specific Technique for the Detection of Collagenase Using Zymography.
In-gel zymography is a commonly employed tool to identify active enzymes in a quantitative and qualitative manner. In this work, apart from the incorporation of substrate which is traditionally… Expand
Obtaining a high activity subtilisin preparation by controlled thermal stress in n-octane.
The use of enzymes in organic solvents has considerably widened their repertoire of applications. Such low water containing media also offer the possibility of carrying out enzymatic reactions at… Expand