Shivasankari Gomathinayagam

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Topoisomerase I (TOP1) inhibitors are an important class of anticancer drugs. The cytotoxicity of TOP1 inhibitors can be modulated by replication fork reversal through a process that requires poly(ADP-ribose) polymerase (PARP) activity. Whether regressed forks can efficiently restart and what factors are required to restart fork progression after fork(More)
Accurate processing of stalled or damaged DNA replication forks is paramount to genomic integrity and recent work points to replication fork reversal and restart as a central mechanism to ensuring high-fidelity DNA replication. Here, we identify a novel DNA2- and WRN-dependent mechanism of reversed replication fork processing and restart after prolonged(More)
RecQ helicases are a widely conserved family of ATP-dependent motors with diverse roles in nearly every aspect of bacterial and eukaryotic genome maintenance. However, the physical mechanisms by which RecQ helicases recognize and process specific DNA replication and repair intermediates are largely unknown. Here, we solved crystal structures of the human(More)
Replicative hexameric helicases are thought to unwind duplex DNA by steric exclusion (SE), where one DNA strand is encircled by the hexamer and the other is excluded from the central channel. However, interactions with the excluded strand on the exterior surface of hexameric helicases have also been shown to be important for DNA unwinding, giving rise to(More)
The Rockefeller University Press $30.00 J. Cell Biol. Vol. 208 No. 5 545–562 www.jcb.org/cgi/doi/10.1083/jcb.201406100 JCB 545 *S. Thangavel and M. Berti contributed equally to this paper. Correspondence to Alessandro Vindigni: avindign@slu.edu Abbreviations used in this paper: CPT, camptothecin; DSB, double-strand DNA break; EXO1, human exonuclease I; HDR,(More)
Strain improvement was carried out to obtain higher chitinase and protein by inter-specific protoplast fusion between Trichoderma harzianum and Trichoderma viride. Fusant HF9 and parental strains of Trichoderma were compared for chitinase and protein production. 1% of glucose, sucrose and fungal cell wall (Rhizoctonia solani), were used as carbon source for(More)
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