Shirley S Pease

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Single-cell mouse embryos were infected in vitro with recombinant lentiviral vectors to generate transgenic mice carrying the green fluorescent protein (GFP) gene driven by a ubiquitously expressing promoter. Eighty percent of founder mice carried at least one copy of the transgene, and 90% of these expressed GFP at high levels. Progeny inherited the(More)
Transcription factors are central to sustaining pluripotency, yet little is known about transcription factor dynamics in defining pluripotency in the early mammalian embryo. Here, we establish a fluorescence decay after photoactivation (FDAP) assay to quantitatively study the kinetic behaviour of Oct4, a key transcription factor controlling pre-implantation(More)
We have identified a myelin basic protein (MBP) isoform in mouse embryos that includes an exon upstream of the usual transcription initiation site. This isoform, embryonic-neonatal MBP (E-MBP), is expressed at the protein level in the embryonic nervous system at a time when other MBP isoforms are not detected. In addition to the central and peripheral(More)
During T cell development, multipotent progenitors relinquish competence for other fates and commit to the T cell lineage by turning on Bcl11b, which encodes a transcription factor. To clarify lineage commitment mechanisms, we followed developing T cells at the single-cell level using Bcl11b knock-in fluorescent reporter mice. Notch signaling and(More)
Rat X rat hybridomas secreting monoclonal anti-idiotypic antibodies have been prepared from Hooded rats immunized with two tumour-reactive, syngeneic monoclonal antibodies 11/160 and M10/76 (specific, respectively, for the Hooded rat sarcomata HSN and MC24). The hybridomas were selected on the basis that the secreted antibodies competed with antigen for(More)
All Rights Reserved iii ACKNOWLEDGMENTS I would like to acknowledge the many collaborators and co-workers who have been extremely helpful to me throughout the course of my graduate studies; I performed virtually none of the work described here entirely by myself. I am endebted to the efforts of many who have helped to start and maintain the transgenic mice,(More)
association with masked and unmasked mRNAs (fig. S5A). b-actin mRNAs hybridized with a second-color probe after protease digestion revealed previously masked mRNAs (about half of the total). Masked mRNAs were associated with brighter ribosomal puncta, supporting the hypothesis that regions rich in ribosomes are part of the same masked complex (Fig. 2C and(More)
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