Shirish Damle

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Monoclonal antibodies (mAbs) (4F2 and 7E12) were prepared against heparan sulfate proteoglycan (HSPG) isolated from bovine glomeruli. Enzyme linked immunosorbent assays (ELISA) and immunoblotting demonstrated that the mABs reacted with HSPG. Indirect immunofluorescence (IF) showed that the mAbs stained renal basement membranes (BMs) and BMs in other organs(More)
Normal human colon crypt protein extract was resolved by two-dimensional gel electrophoresis using pH 6-11 immobilized pH gradient strips in the first dimension. The optimized isoelectric focusing protocol includes cup-loading sample application at the anode and 1.2% hydroxyethyl disulfide (DeStreak), 15% 2-propanol and 5% glycerol in the rehydration(More)
Twenty-six monoclonal antibodies (MAbs) developed against rabbit corneal proteokeratan sulfate (PKS), were used to evaluate immunohistochemically the ocular distribution of PKS during prenatal and early postnatal development in rabbits. These MAbs were directed against epitopes located in the keratan sulfate (KS) chains of the proteoglycan (SundarRaj et(More)
cell lines, whereas alkaline phosphohydrolase activity was 8 times greater in Sarcoma 180/TG. Alkaline phosphohydrolase activity was localized predominantly in particulate fractions from Sarcoma 180/TG, showed a pH optimum of 9.2, and hydrolyzed a wide variety of phosphate esters, including p-nitropheny (phosphate and 5'-nucleotides, such as 6-thioinosine(More)
Streptococcus faecalis subsp. zymogenes strain DS16 was found to harbor two plasmids, designated pAD1 (35 megadaltons) and pAD2 (15 megadaltons). pAD1 is transmissible and determines a hemolysin-bacteriocin, whereas pAD2 is non-conjugative and determines resistance to erythromycin, streptomycin, and kanamycin. pAD2 could be mobilized by pAD1, but usually(More)
The major proteoglycan of pig skin, a proteodermatan sulfate, has been isolated under mild conditions in the presence of protease inhibitors. After purification by ion exchange and gel chromatography, it has a Mr of 70 x 10(3) and it does not form aggregates with hyaluronic acid. It contains about 60% protein, one chain of dermatan sulfate, and several(More)
Proteoglycans extracted from rabbit corneal stroma can be separated by ion exchange and gel chromatography into two proteokeratan sulfates (PKS-I, PKS-II) and two proteodermatan sulfates (PDS-I, PDS-II). PKS-I (21% of the total glycosaminoglycans) contains 48% protein, and PKS-II (43% of the total) has 57% protein. In both, the only hexosamine is(More)
Hybridomas were developed that secreted monoclonal antibodies against two proteokeratan sulfates (PKS) from rabbit corneal stroma. A total of 28 antibodies were isolated, all of the IgG type with kappa light chains. All were found to react with both PKSs. As judged by immunohistochemical staining, none of them reacted with scleral or conjunctival(More)
Macromolecules from normal rabbit cornea and cornea containing a 2-mm diameter button of scar tissue were biosynthetically labeled with 35S-sulfate and 3H-glucosamine in vivo and in organ culture. Labeled macromolecules, including proteoglycans (PGs) extracted from the normal cornea, scar tissue, and corneal tissue adjacent to the scar with guanidine(More)