Shiori Sugamata Aki

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Following meiosis, plant gametophytes develop through two or three rounds of mitosis. Although the ontogeny of gametophyte development has been defined in Arabidopsis thaliana, the molecular mechanisms regulating mitotic cell cycle progression are not well understood. Here, we report that RING-H2 group F 1a (RHF1a) and RHF2a, two RING-finger E3 ligases,(More)
Flowering plants produce multicellular gametophytes through an elaborate regulation of gametogenesis. During female and male gametogenesis in Arabidopsis thaliana, sporogenous cells differentiate and undergo meiosis to produce megaspores and microspores, which in turn go through mitosis to develop into multicellular gametophytes. Here we report that the(More)
The regulation of protein turnover by the ubiquitin proteasome system (UPS) is a major posttranslational mechanism in eukaryotes. One of the key components of the UPS, the COP9 signalosome (CSN), regulates 'cullin-ring' E3 ubiquitin ligases. In plants, CSN participates in diverse cellular and developmental processes, ranging from light signaling to cell(More)
Jingjing Liu,a Yiyue Zhang,b Genji Qin,a Tomohiko Tsuge,c Norihiro Sakaguchi,d Guo Luo,a Kangtai Sun,a Dongqiao Shi,b Shiori Aki,c Nuoyan Zheng,b Takashi Aoyama,c Atsuhiro Oka,c Weicai Yang,b Masaaki Umeda,d Qi Xie,b Hongya Gu,a,e and Li-Jia Qua,e,1 a National Laboratory for Protein Engineering and Plant Genetic Engineering, Peking-Yale Joint Research(More)
This chapter focuses on visualization of cell cycle progression in plant tissues using the dual-color marker system "Cytrap." The Cytrap line carries a part of Arabidopsis CDT1a fused to the red fluorescent protein (RFP) gene, which monitors the cell cycle phases from S to late G2 or early mitosis, and the G2/M-specific cyclin B1 marker fused to the green(More)
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