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A novel transforming growth factor-beta superfamily member expressed in gonadal somatic cells enhances primordial germ cell and spermatogonial proliferation in rainbow trout (Oncorhynchus mykiss).
Generation of functional eggs and sperm from cryopreserved whole testes
- Seungki Lee, Y. Iwasaki, Shinya Shikina, G. Yoshizaki
- Biology, Environmental ScienceProceedings of the National Academy of Sciences
- 14 January 2013
Fertilization of resultant gametes resulted in the successful production of normal, frozen ASG-derived offspring, and feasibility and simplicity of this methodology will call for an immediate application for real conservation of endangered wild salmonids.
Production of Trout Offspring from Triploid Salmon Parents
- T. Okutsu, Shinya Shikina, Megumi Kanno, Y. Takeuchi, G. Yoshizaki
- Biology, Environmental ScienceScience
- 14 September 2007
By transplanting cryopreserved spermatogonia from rainbow trout into sterile xenogeneic recipients, this study can generate individuals of a threatened species by transplanted into newly hatched sterile triploid masu salmon.
Culture conditions for maintaining the survival and mitotic activity of rainbow trout transplantable type A spermatogonia
The cultured spermatogonia could colonize and proliferate in recipient gonads following transplantation and represents the first step towards establishing a cell line that can be transplanted for use in surrogate broodstock technology and cell‐mediated gene‐transfer systems.
Sexual plasticity of ovarian germ cells in rainbow trout
It is shown here that transplanted ovarian germ cells isolated from 6- to 9-month-old donors can colonize sexually undifferentiated embryonic gonads and resume gametogenesis and indicate that mitotic germ cells, the oogonia, possess a high level of sexual plasticity.
Spermatogonial transplantation in fish: A novel method for the preservation of genetic resources.
Production of germ cell‐deficient salmonids by dead end gene knockdown, and their use as recipients for germ cell transplantation
Dead end‐knockdown salmonids appear to be ideal recipients for the intraperitoneal transplantation of spermatogonia in rainbow trout, and the donor‐derived germ cells showed significantly higher proliferation in the gonads of germ cell‐deficient recipients than those in the Gonads of the control recipients.
Manipulation of fish germ cell: visualization, cryopreservation and transplantation.
The first germ-cell transplantation system in lower vertebrates using fish PGCs and spermatogonia is developed, which allows the donor germ cells to migrate towards, and subsequently colonize, the recipient genital ridges.
Yolk formation in a stony coral Euphyllia ancora (Cnidaria, Anthozoa): insight into the evolution of vitellogenesis in nonbilaterian animals.
The characteristics of Vg that were identified in corals were somewhat similar to those of VG in bilaterian oviparous animals, raising the hypothesis that such characteristics were likely present in the oogenesis of some common ancestor prior to divergence of the cnidarian and bilateralian lineages.
Lymphocyte Antigen 75 (Ly75/CD205) Is a Surface Marker on Mitotic Germ Cells in Rainbow Trout1
Lymphocyte antigen 75 (Ly75/CD205) was identified as a germ cell-specific cell surface marker by combination expressed sequence tag analysis of purified type A spermatogonia from immature testis, in silico prediction of membrane proteins, and expression studies.