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We have constructed a chimeric gene consisting of the promoter, first exon, and first intron of a maize ubiquitin gene (Ubi-1) and the coding sequence of the bar gene from Streptomyces hygroscopicus. This construct was transferred into rice (Oryza sativa L.) protoplasts via electroporation, and 10 plants were regenerated from calli that had been selected(More)
The gravimetric determination of selenium, by reduction of selenious acid in perchloric acid solution with hydrazine hydrate, has been studied in detail. Elemental selenium is completely precipitated in 3.2-5.1M perchloric acid at room temperature or 100 degrees C. One mole of selenious acid reacts with one mole of hydrazine hydrate to give elemental(More)
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