Shinobu Watarai

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The usefulness of pH-sensitive fusogenic polymer-(succinylated poly(glycidol)-(SucPG-) modified liposomes as a vaccine carrier in the induction of immune responses was evaluated. Mice were intraperitoneally immunized with ovalbumin- (OVA-) containing SucPG-modified liposomes. After immunization, significant OVA-specific antibodies were detected in the(More)
Clostridium botulinum type B neurotoxin (BoNT/B) recognizes a complex of synaptotagmin II and ganglioside GT1b or GD1a as the high-affinity toxin binding site. Recombinant deletion mutants of synaptotagmin II allowed us to demonstrate that the N-terminal domain including the transmembrane region retains BoNT/B binding activity while the C-terminal domain is(More)
On the basis of the results outlined in our previous report, bacterial sialyltransferases (ST) from marine sources were further characterized using glycosphingolipids (GSL), especially ganglio-series GSLs, based on the enzymatic characteristics and kinetic parameters obtained by Line weaver-Burk plots. Among them, GA1 and GA2 were found to be good(More)
Astrocytes are the cells that actively participate in the process of lesion repair in the central nervous system (CNS), and reactive astrocytosis of varying degrees becomes apparent with time in any pathological condition occurring in the normally developed postnatal CNS. Ganglioside GD3 (II3a(NeuAca2-8NeuAc)-LacCer, GD3) in reactive astrocytes from(More)
A monoclonal antibody (MAb), generated by immunizing BALB/c mice with homogenized bovine retinal tissue, was specific to ganglioside GD1b incorporated into liposome membranes. The antibody (MAb-5G6), classified as IgM, immunostained intensely the perikaryon and processes of motoneurons in the cranial motor nuclei and spinal cord. Spinal and trigeminal(More)
Immunohistochemical staining for GD3 in adult rat brain was carried out using mouse IgM anti-GD3 monoclonal antibody (DSG-1). Neuronal cells in the cerebral cortex, striatum, hippocampus and various nuclei of the thalamus were immunoreactive. In the cerebellum, Purkinje cells, granule cells and also basket cells were immunoreactive. However, astrocytes and(More)
Immunocytochemical staining for ganglioside GD3 (II3 alpha(Neu Ac alpha 2-8 NeuAc)-LacCer) in the choroid plexus of the adult rat brain was carried out. A peroxidase reaction product for GD3 was predominantly present along the surface of microvilli of the choroidal cells, suggesting that ganglioside GD3 may be concerned with the formation or secretion of(More)
Light and electron microscopy observations were made of developing rat brain at gestation days (E) E13, E16, E19, and postnatal days (P) P1, P3, P5, P7 after immunocytochemical staining for ganglioside GD3 (II3 alpha(NeuAc alpha 2-8NeuAc)-LacCer, GD3) using mouse IgM anti-GD3 monoclonal antibody (DSG-1). Immunoreactivity was observed in neuroblasts (E13,(More)
Immunocytochemical study of ganglioside GD3 (II3 alpha(NeuAc alpha 2-8NeuAc)-LacCer) was performed in the cold lesions produced in the cerebral cortex of the rat brain, using mouse IgM anti-GD3 monoclonal antibody (DSG-1). Seven and 15 days after cold lesioning, GD3-like immunoreactivity was observed in reactive astrocytes. Thirty and 50 days after cold(More)
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