Shinji Sugiura

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Cell sorting is an essential and efficient experimental tool for the isolation and characterization of target cells. A three-dimensional environment is crucial in determining cell behavior and cell fate in biological analysis. Herein, we have applied photodegradable hydrogels to optical cell separation from a 3D environment using a computer-controlled light(More)
This paper describes the generation of "click-crosslinkable" and "photodegaradable" gelatin hydrogels from the reaction between dibenzocycloctyl-terminated photoclevable tetra-arm polyethylene glycol and azide-modified gelatin. The hydrogels were formed in 30 min through the click-crosslinking reaction. The micropatterned features in the hydrogels were(More)
Introduced species negatively impact native species through competitive and trophic interactions, particularly on oceanic islands that have never been connected to any continental landmass. However, there are few studies on the relative importance of competitive interactions (resource competition with introduced species) and trophic interactions (predation(More)
Some animals have evolved the use of environmental materials as "portable armour" against natural enemies. Portable bags that bagworm larvae (Lepidoptera: Psychidae) construct using their own silk and plant parts are generally believed to play an important role as a physical barrier against natural enemies. However, no experimental studies have tested the(More)
The eastern hive bee Apis cerana is a major honeybee species in Asia providing numerous ecosystem services. Understanding how much the honeybees depend on natural and human-influenced plants and landscapes in different climates is important could contribute to evaluate how wild honeybees use food resources and to measure the ecosystem services. We(More)
Resource subsidies are well known to increase population densities of consumers. The decomposition process of these subsidised resources can be influenced by increasing consumer abundance. However, few studies have assessed whether resource subsidies can promote resource decomposition via a population increase in consumers. Here, we examined the effects of(More)
We designed specific primers for Coniothyrium minitans, a sclerotia-parasitizing biocontrol agent (BCA), and quantified its biomass in the field by quantitative polymerase chain reaction. Because chemical properties of the soil can decrease the DNA extraction efficiency and DNA polymerase activity, standard curves were calibrated by adding known(More)
Herons (Ardeidae) frequently breed in inland forests and provide organic material in the form of carcasses of prey (that they drop) and chicks (that die) to the forest floor. Such allochthonous inputs of organic materials are known to increase arthropod populations in forests. However, the exact species that show numerical responses to allochthonous inputs(More)