- Full text PDF available (14)
This paper reports a serial dilution microfluidic network composed of microchannels with a high fluidic-resistance ratio for generating linear concentration profiles as well as logarithmic concentration profiles spanning 3 and 6 orders of magnitude. The microfluidic networks were composed of thin fluidic-resistance microchannels with 160 to 730 microm(2)… (More)
Copris ochus (Coleoptera: Scarabaeidae), an endangered species, is the largest dung beetle in Japan. In C. ochus, males have a long head horn, while females lack this long horn (sexual dimorphism). Very large males of C. ochus have disproportionately longer head horns than small males, suggesting male horn dimorphism, although the dimorphism has not been… (More)
Cell sorting is an essential and efficient experimental tool for the isolation and characterization of target cells. A three-dimensional environment is crucial in determining cell behavior and cell fate in biological analysis. Herein, we have applied photodegradable hydrogels to optical cell separation from a 3D environment using a computer-controlled light… (More)
Novel on-chip fluid control strategies, on-demand formation of arbitrary microchannels and parallel control of multiple microvalves were successfully demonstrated by means of computer-controlled micropatterned light irradiation of a photoresponsive hydrogel sheet.
This paper describes the generation of "click-crosslinkable" and "photodegaradable" gelatin hydrogels from the reaction between dibenzocycloctyl-terminated photoclevable tetra-arm polyethylene glycol and azide-modified gelatin. The hydrogels were formed in 30 min through the click-crosslinking reaction. The micropatterned features in the hydrogels were… (More)
The decomposition of vertebrate carcasses is an important ecosystem function. Soft tissues of dead vertebrates are rapidly decomposed by diverse animals. However, decomposition of hard tissues such as hairs and feathers is much slower because only a few animals can digest keratin, a protein that is concentrated in hairs and feathers. Although beetles of the… (More)
Native vegetation is frequently replaced by alien plants on isolated oceanic islands. The effects of such replacements by invasive plants on the diversity and temporal dynamics of island-endemic insects remain unclear. We examined flying insect communities using Malaise traps on the small island of Nishi-jima in the oceanic Ogasawara Archipelago in the… (More)
We have developed a microarray of cell culture environments composed of a combination of soluble factors and extracellular matrices for screening of cell culture environment.
We developed a novel microfluidic device to prepare monodisperse water-in-oil-in-water (W/O/W) emulsions with an ultra-thin (<1 microm) oil phase layer. This microfluidic device was composed of two microchannel junctions, one of which had a step structure, and a uniformly hydrophobic surface for effective oil removal from W/O/W droplets. At the first… (More)
Consistent liver metastases in animal models is generally observed only with certain cancer cell lines. With the aim of improving on existing animal models of liver metastases, we hypothesized that cancer cells encased in 300 microm microcapsules, mimicking micrometastatic foci, might be effective seeds of liver metastases. A total of 3,000 microcapsules,… (More)