Shinichiro Kobayashi

Learn More
We constructed a pTD1 vector for an insect cell-free translation system containing a 5' untranslated region (UTR) of a polyhedrin gene as a translational enhancer sequence. Its translational efficiency was about 50-fold higher than those of mRNAs without an enhancer sequence. Moreover, the pTD1 vector functioned as an effective expression vector not only in(More)
The p53 tumor suppressor protein induces cell cycle arrest or apoptosis in response to cellular stresses. We have identified PRG3 (p53-responsive gene 3), which is induced specifically under p53-dependent apoptotic conditions in human colon cancer cells, and encodes a novel polypeptide of 373 amino acids with a predicted molecular mass of 40.5 kDa. PRG3 has(More)
Ferritin is a class of iron storage protein composed of 24 subunits. Although many studies on gene expression analyses of plant ferritin have been conducted, the functions and oligomeric assembly of plant ferritin subunits are still largely unknown. In order to characterize the ability to form multimeric protein shells and determine the iron incorporating(More)
BACKGROUND/AIMS The prognosis of some patients with gastric tumors that have infiltrated adjacent organs may be improved by operations that include complete removal of these structures. However, such a radical operation raises concerns about operative risk. METHODOLOGY We performed a retrospective analysis of 55 patients whose gastric primary tumors(More)
Endoscopic mucosal resection (EMR) and endoscopic submucosal dissection (ESD) have recently been accepted as less invasive methods for treating patients with early esophageal cancers such as squamous cell carcinoma and dysplasia of Barrett's esophagus. However, the large defects in the esophageal mucosa often cause severe esophageal strictures, which(More)
Indomethacin, a common nonsteroidal anti-inflammatory drug, has been shown to enhance radiation-mediated cell-killing effect through the activation of p38 mitogen-activated protein kinase (MAPK). We found that indomethacin strongly reduced the basal level of extracellular signal-regulated kinases 1 and 2 (ERK1/2) in HT-29 human colon carcinoma cells. The(More)
More than 50% of human cancers contain p53 gene mutations and as a result accumulate altered forms of the full-length p53 protein. Although certain tumor types expressing mutant p53 protein have a poor prognostic process, the precise role of mutant p53 protein in highly malignant tumor cells is not well defined. Some p53 mutants, but not wild-type p53, are(More)
A randomized study design was used to compare the efficacy of cefotiam (CTM) and fosfomycin (FOM) for preventing infection associated with urologic surgery. Of 207 patients initially enrolled, data from 202 were evaluated for drug safety, and data from 195 were evaluated for efficacy (115 transurethral endoscopic surgeries, 20 clean surgeries, 54(More)
BACKGROUND/AIMS Because the HAMA (human anti-mouse antibody) response following administration of murine monoclonal antibodies represents mainly isotypic HAMA production, idiotypic HAMA responses have not been thoroughly analyzed. METHODOLOGY In the present study we examined the effect of idiotypic HAMA that arose in patients who had repeatedly received(More)
BACKGROUND/AIMS One problem that has been encountered with the use of murine Mabs is the development of a human anti-mouse antibody (HAMA) which lead to serious problems such as anaphylaxis. In order to evaluate the decreased HAMA production after administration of Fab fragments of chimeric monoclonal antibody, Fab fragments of chimeric Mab A7 (chA7Fab) was(More)