Shinichi Fukushige

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Germ-line mutations in the hMLH1 gene are the most frequent cause of hereditary nonpolyposis colorectal cancer and are characterized by missense mutations at high frequency. We found a yeast two-hybrid assay to be an extremely useful and simple tool for evaluating the biological significance of such hMLH1 germ-line missense mutations; 78% (18 of 23) of the(More)
In human cells, hMLH1, hMLH3, hPMS1 and hPMS2 are four recognised and distinctive homologues of MutL, an essential component of the bacterial DNA mismatch repair (MMR) system. The hMLH1 protein forms three different heterodimers with one of the other MutL homologues. As a first step towards functional analysis of these molecules, we determined the(More)
not required for the expression of transgenes, its inactivation also did not restore detectable expression of rnr::GFP in cyd-1 mutants. However, we expect that lin-36; cyd-1 double mutant larvae express Loss of dpl-1 Represses rnr::GFP Expression A potential caveat of examining the effect of loss of dpl-1 on rnr::GFP the endogenous rnr-1 gene at a low(More)
Two erbA homologs, termed ear-1 and ear-7, are present in the human genome on chromosome 17. The two genes reside in the same genetic locus with overlapping exons and are transcribed from opposite DNA strands. In addition, the ear-7 mRNA is alternatively spliced to generate two protein isoforms, namely the ear71 and ear72 proteins. Nucleotide sequence(More)
We have analysed the loss of heterozygosity (LOH) on chromosome bands 16q22-q24 in 24 primary gastric cancer tissues and found three regions of frequent allelic loss (16q22, 16q24.1-q24.3 and 16q24.3). The region for the most frequent allelic loss (63%) was in 16q24.1-q24.3. LOH of this region had no relationship with histological subtype, but a significant(More)
Cytogenetic and molecular studies demonstrated that pancreatic cancer frequently shows specific chromosomal abnormalities, such as losses of 9p, 17p, and 18q, and gains of 8q and 20q. We have analyzed alterations in the copy number of specific chromosomal regions in cells from the pancreatic juices of 32 patients with various pancreatic disorders by(More)
One of the challenges in human genome research is to identify the promoter sequences which play a key role in the regulation of gene expression. We report here a new promoter trapping system for use with mammalian cells comprised of the following three steps: 1) Cloning of DNA fragments into a promotertrapping vector, 2) integration of the trapping vector(More)
Visibility determination is one of the oldest problems in computer graphics. The visibility, in terms of back-to-front polygon visibility ordering, can be determined by updating a priority list as the viewpoint moves. A new list-priority algorithm, utilizing a property of Voronoi diagrams, is proposed in this paper. In the preprocessing phase, the 3D space(More)
We propose a system that uses a camera and a mirror to input behaviour of a pointer in 3D space. Using direct and reflection images of the pointer obtained from single directional camera input, the system computes the 3D positions and the normal vector of the mirror simultaneously. Although the system can only input the "relative positions" of the pointer,(More)
Life cycle design is a promising approach for introducing efficient resource circulation. In such design, there are difficulties in balancing demand and supply for resources throughout product life cycles. For the resource balance, it is important to design a product life cycle focusing on individual products and their parts, since they change their states(More)