Shingo Hotta

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R. Alberto Travagli The nucleus tractus solitarius: an integrative centre with ‘task-matching’ capabilities J Physiol 2007 582: 471. First Published online on May 31, 2007 D. A. Giussani Hypoxia, fetal growth and early origins of disease: the Andean curse on the Conquistadors J Physiol 2007 582: 472. First Published online on June 7, 2007 Stefan Kääb(More)
Intracellular Ca(2+) ([Ca(2+)](i)) oscillations seen in interstitial cells of Cajal (ICCs) are considered to be the primary pacemaker activity in the gut. Here, we show evidence that periodic Ca(2+) release from intracellular Ca(2+) stores produces [Ca(2+)](i) oscillations in ICCs, using cell cluster preparations isolated from mouse ileum. The pacemaker(More)
Hisao Yamamura,* William C. Cole,* Satomi Kita, Shingo Hotta, Hidemichi Murata, Yoshiaki Suzuki, Susumu Ohya, Takahiro Iwamoto, and Yuji Imaizumi Department of Molecular and Cellular Pharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, Japan; The Smooth Muscle Research Group, Department of Physiology and Pharmacology,(More)
The possibility that the ryanodine receptor type 2 (RyR2) can function as the major Ca(2+)-induced Ca(2+) release (CICR) channel in excitation-contraction (E-C) coupling was examined in smooth muscle cells (SMCs) isolated from urinary bladder (UB) of RyR2 heterozygous KO mice (RyR2+/-). RyR2 mRNA expression in UB from RyR2+/- was much lower than that in(More)
The Na(+)/Ca(2+) exchanger (NCX) is thought to be a key molecule in the regulation of cytosolic Ca(2+) dynamics. The relative importance of the two Ca(2+) transport modes of NCX activity leading to Ca(2+) efflux (forward) and influx (reverse) in smooth muscle, however, remains unclear. Unexpectedly, spontaneous contractions of urinary bladder smooth muscle(More)
We examined the effects of methyl-beta-cyclodextrin (MbetaCD) on Ca(2+)-induced Ca(2+) release (CICR) in smooth muscle cells (SMCs) of mouse urinary bladder (UB). Short depolarization of UBSMCs under voltage-clamp elicited several local Ca(2+) transients (Ca(2+) hot spots) via CICR within 20 ms in discrete sub-sarcolemmal areas. Then, the Ca(2+) wave spread(More)
The rise of Ca(2+) concentration ([Ca(2+)](i)) by reducing external Na(+) in urinary bladder smooth muscle cells (UBSMCs) from transgenic mice overexpressing Na(+)/Ca(2+) exchanger type-1.3 (NCX1.3(tg/tg)) was about 4 times as large as that in the wild-type (WT). NCX1 protein expression in UB increased about 4-fold in NCX1.3(tg/tg). The Ca(2+) release by(More)
The activated B-cell diffuse large B-cell-like lymphoma (ABC-DLBCL) correlates with poor prognosis. The B-cell receptor signaling pathway is known to be dysregulated in NHL/CLL and given BTK is a downstream mediator of BCR signaling, BTK constitutes an interesting and obvious therapeutic target. Given the high potency and selectivity of the BTK inhibitor,(More)
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