Shin-ichiro M. Nomura

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The cellular environment differs from that of reconstituted materials mainly because of the presence of highly condensed biomacromolecules. To mimic the environment and conditions in living cells, we developed a method to prepare additive-free, highly concentrated cell extracts. First, we verified the requirement for specific salts and buffers for(More)
Replication of all living cells relies on the multirounds flow of the central dogma. Especially, expression of DNA replication proteins is a key step to circulate the processes of the central dogma. Here we achieved the entire sequential transcription-translation-replication process by autonomous expression of chromosomal DNA replication machineries from a(More)
Molecular communication [1]-[2] is an emerging communication paradigm that uses molecules as a communication medium. Molecular communication allows biological and artificially created nano- or cell-scale devices to communicate with each other. In molecular communication, senders encode information onto molecules (called information molecules), and(More)
Here, we report a method for introducing large objects of up to a micrometer in diameter into cultured mammalian cells by electrofusion of giant unilamellar vesicles. We prepared GUVs containing various artificial objects using a water-in-oil (w/o) emulsion centrifugation method. GUVs and dispersed HeLa cells were exposed to an alternating current (AC)(More)
Toward reconstitution of living cells by artificial cells technology, it is critical process to understand the differences between mixtures of biomolecules and living cells. For the aim, we have developed procedures for preparation of an additive-free cell extract (AFCE) and for concentrating biomacromolecules in artificial cells. In this review, we(More)
Prototype artificial cell models with designed functional molecules are presented here. Artificial molecular devices based on a giant liposome were prepared to obtain specific properties that cannot be obtained from natural cells. In this context, artificial cell research is seen an extension of " molecular robotics " research. Cooperative and integrated(More)
We aimed to develop a micro-robot that can crawl on contact surfaces in biological environments. The prototype chassis of this micro-bot consists of a lipid membrane that encapsulates and bonds micro-sized magnetic particles. By applying a rotating magnetic field, we hope to obtain a micro-crawler robot. In this report, we describe our observations of the(More)
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