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PURPOSE To identify the genotype of 18S ribosomal RNA gene (18S rDNA, Rns) of the Acanthamoeba strains isolated from patients with keratitis in northern China. METHODS The genus-specific primers JDP1 and JDP2 were used for the amplification of the amplimer ASA.S1. With DNA PCR and sequencing, Rns genotypes were identified according to the DF3 sequence(More)
OBJECTIVE To study on the distribution of bacterial keratitis isolates and the resistance to antibiotics in China from 2001 to 2004. METHODS 1985 specimens from the bacterial keratitis at the Beijing Tong Ren Eye Center were cultured and identified. In vitro susceptibility testing of positive isolates to antibiotics was determined by the Kirby-Bauer disk(More)
OBJECTIVE To review the clinical characteristics, treatment, and outcome of patients with Acanthamoeba keratitis (AK). DESIGN Retrospective, noncomparative, interventional case series. PARTICIPANTS Twenty consecutive patients with AK were referred to the Beijing Tongren Hospital, Capital University of Medical Science, China. METHODS Retrospective(More)
PURPOSE To report 28 cases of infectious keratitis related to orthokeratology lens overnight wear in China. METHODS From March 2000 to August 2001, 28 cases of infectious keratitis related to overnight orthokeratology lens wear were diagnosed in Beijing Institute of Ophthalmology. These were retrospectively reviewed with regard to the pathogens isolated,(More)
PURPOSE We compared the regeneration characteristics of injured corneal subbasal nerve fibers (CSNFs) and keratocyte reaction following LASIK with a femtosecond laser (FS-LASIK), One Use-Plus Sub-Bowman's keratomileusis (OUP-SBK), and M2 90 microkeratome to provide a more suitable treatment strategy. METHODS A total of 22 eyes that underwent FS-LASIK, 32(More)
BACKGROUND Due to differences in corneal grafting microsurgery between manual and robotic suturing, new challenges have arisen in testing the insertion force and torque of corneal tissue acting on suturing needles in order to guarantee successful completion of surgical procedures. METHODS In order to measure the force during the insertion operation, from(More)
The aim of this study was to investigate the etiological characteristics of Chlamydia trachomatis conjunctivitis among resident students at primary schools in the Qinghai Tibetan area in order to understand the distribution of C. trachomatis and other pathogenic microorganisms, to detect the isolation rate of infectious pathogens, and to provide an evidence(More)
Trachoma is one of the most widespread blinding eye diseases, which is harmful to human visual health. The efforts to prevent and control trachoma in China can be divided into three periods, i.e., highly epidemic period, research on pathogenesis and control of trachoma, and blinding trachoma elimination and epidemiological evaluation. In 1956, Prof. Feifan(More)
There has been substantial progress in our understanding of the ocular surface system/lacrimal function unit in the past 15 years. Keratoconjunctivitis sicca, more commonly referred to as dry eye syndrome (DES), is the most frequently encountered condition and diabetes mellitus (DM) has been identified as one of the leading causes of DES. Poor glycemic(More)
Morbidity associated with conjunctivitis is higher in developing countries, particularly among children, because of the poorer standards of living (Azari and Barney, 2013; Yetman and Coody, 1997). Presently, little is known regarding the microorganisms that cause conjunctivitis in the remote area of Chinese Tibet. In particular, it is difficult to monitor(More)