Shih-Chieh Tsai

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Members of the p160 nuclear receptor coactivators interact with liganded nuclear receptors to enhance transcription of target genes. Here we identify a novel family of ankyrin repeats containing cofactors (ANCOs) that interact with the p160 coactivators. ANCO-1 binds to the conserved Per-Arnt-Sim (PAS) region of the p160 coactivators. It encodes a large(More)
Known spectroscopic and kinetic data are used to formulate pathways of the physiological and transfer reactions and the substrate inhibition of phenol sulfotransferase. Kinetic mechanisms indicate that release of PAP from enzyme complex is required for the physiological reaction but not for the transfer reaction. The pathways explain rate difference between(More)
We demonstrate a high-power passively Q -switched and mode-locked Nd:YVO(4) laser with a Cr(4+):YAG saturable absorber. 2.7 W of average power with an 18-kHz Q -switched repetition rate was generated at a 12.5-W pump power. The peak power of a single pulse near the maximum of the Q -switched envelope was greater than 100 kW.
Monoclonal antibody complexes have proven very useful in the study of low-density lipoproteins (LDLs). Thus, complexes composed of two different monoclonal antibodies, selected from a panel of 11 different antibodies, and LDL have been employed to map apolipoprotein B (apoB) on the surface of the LDL. In this way, apoB was found to surround the LDL as a(More)
  • Yung-Fu Chen, S W Tsai
  • 2002
A compact high-power yellow pulsed laser has been demonstrated by use of intracavity sum-frequency mixing in a diode-end-pumped Q -switched Nd:YVO(4) dual-wavelength laser. A three-mirror configuration forming two separate laser cavities is used to optimize the gain match for simultaneous dual-wavelength emission in Q -switched operation. Under the optimum(More)
We demonstrate a high-power passively mode-locked Nd:YVO (4) laser that uses a saturable Bragg reflector (SBR) with strain relaxation. 23.5 W of average power with ~21.5-ps cw mode-locked pulse trains was generated at a 50-W pump power. Experimental results show that appropriate strain relaxation in the SBR makes the mode-locking operation less sensitive to(More)
An enantioselective esterification process was developed for the synthesis of 2-N-morpholinoethyl (S)-ibuprofen ester prodrug from racemic ibuprofen by using Candida rugosa lipase immobilized on Accurel MP1000 in cyclohexane. Compared with the performance of Lipase MY, the immobilized lipase possesses a higher enzyme activity and thermal stability, but with(More)
Daxx plays a major role in several important signaling pathways including transcription and cell death. It has been postulated that Daxx regulates both events from the nucleus; however, the mechanism by which Daxx is localized in the nucleus remains obscure. Here we show that nuclear localization of Daxx is controlled by two independent signals and importin(More)
A 159 residue, N-terminal fragment of the human C1s complement component, C1s alpha(159), was expressed in the baculovirus, insect cell system. The protein was abundantly produced 3 days after infection, reaching levels as high as 40 microg/ml in cell culture media. It had a molecular weight of 18,100 (+/-4.9) Da by laser desorption mass spectrometry, close(More)
The ankyrin repeats cofactor-1 (ANCO-1) was recently identified as a novel nuclear receptor corepressor that regulates receptor-mediated transcription through interactions with p160 coactivators and histone deacetylases. Interestingly, exogenously expressed ANCO-1 is localized at distinct subnuclear domains. The relevance of these subnuclear domains and the(More)