Shigeru Tsuji

Learn More
The adult electric organ of the mormyrid Pollimyrus isidori consists of four longitudinal tubes, two dorsal and two ventral ones, entirely filling the space in the caudal peduncle, as in other mormyrids. Each tube comprises about 100 electrocytes possessing a large innervated stalk just behind their posterior faces. The numerous nerve terminals are deeply(More)
In the original Karnovsky and Roots' method for the localization of acetylcholinesterase (AChE), thiocholine reduces the ferricyanide and cupric ions of this medium competitively, giving simultaneously cupric ferrocyanide (Karnovsky's precipitate) and cuprous thiocholine iodide (Koelle's precipitate) as histochemical products. We modified the method in(More)
In order to carry out ultrastructural immunocytochemical localization of acetylcholinesterase at cholinergic synapses, ultrathin frozen sections of aldehyde-fixed electric organs of Electrophorus and Torpedo were obtained by a modified ultracryotome technique. Dry frozen sections, picked up with rabbit serum and negatively stained, revealed, in the(More)
Immunohistochemical localization of substance P (SP), CGRP, VIP, neuropeptide Y (NPY), and somatostatin (SOM) in the carotid labyrinth were compared in some species of amphibians using the peroxidase-antiperoxidase method. Immunoreactivity of SP, CGRP, VIP, and NPY was found in the nerve fibers distributed in the intervascular stroma of the carotid(More)
Using a glutaraldehyde-fixed mouse neuromuscular junction, a fine precipitate of copper thiocholine was obtained with Koelle's medium prepared by a mixture of phosphate buffer (pH 5.6–5.9) and copper glycine solution (strongly acidic). The final pHs of these incubation media were very low, being situated between 3.8 and 4.2, respectively. It is well known(More)
During a historical research, we realized that René Couteaux (1909-1999) was the first histologist who stained the postsynaptic structure of the neuromuscular junction. By means of Janus Green B dye, he revealed the membranous 'subneural apparatus' related to the 'synaptic gutter'. Hence, he justified the use of the physiological term 'synapse' in(More)
The percutaneous absorption of bromhexine (BH), an expectorant drug, through rat skin was examined in vitro and in vivo. BH in free base form penetrated better than the hydrochloride through the skin. When the in vitro penetration of BH was compared using Plastibase, macrogol and sucrose ester of fatty acid F-160 (DK-ester) formulations, the DK-ester(More)
The treatment of neuromuscular junctions with phosphomolybdic acid (PMA) and silicotungstic acid (STA) heteropolyanions permits the visualization of electron dense precipitates in the synaptic vesicles of the cholinergic motor nerve terminals. At the light microscopic level, the uncolored molybdenum salt is visualized after reduction to molybdenum blue. The(More)
The electromotor neurons (EMNs) of the adult electric organ of Pollimyrus isidori and the "giant neurons" (GNs) found in the more rostral part of the spinal cord were investigated both with light and electron microscopical methods. The two kinds of neuron are more or less similar in size, about 30 microns, and are found in the more dorsal part of the spinal(More)