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The C-banding pattern, location of telomere sequence and chiasma frequency of four species of the Schistosoma japonicum complex were compared with those of two African species, Schistosoma mansoni and Schistosoma haematobium. In the six species, C-banding patterns of seven autosomes and the two sex chromosomes (Z and W) showed relatively species-specific(More)
Human cases of paragonimiasis have been reported in Lao People's Democratic Republic from several provinces, however, detailed descriptions of a focus of transmission including parasites species and intermediate hosts are lacking. Field investigations were carried out between February and July 2004 in three neighboring villages of the province of Vientiane.(More)
Paragonimosis is an important food-borne zoonosis especially in Asian countries. Among Paragonimus species, Paragonimus westermani followed by P. skrjabini complex are the major pathogens for human paragonimosis in Asia. In addition, P. heterotremus is an important pathogen in southern China and the Indochina Peninsula and is the only proven species to(More)
The transcriptional products of Fasciola gigantica genes encoding cathepsin B proteases were cloned from adult, newly excysted juvenile (NEJ), and metacercarial stages. The obtained cDNAs were named FG cat-B1, FG cat-B2, and FG cat-B3. The deduced amino acid sequences of the encoded proteases have identities ranging from 64 to 79%. Sequence comparison with(More)
Based on morphology of metacercariae and adult worms together with molecular data from our previous study, we describe herein a new lung fluke species named Paragonimus vietnamensis sp. nov. Metacercariae of this new species is round and extremely large in size (nearly 800 mum in diameter) having a fragile outer cyst of variable thickness and a thin fragile(More)
During an epidemiological survey for Paragonimus and paragonimosis in northern Vietnam, we found extremely large excysted metacercariae (2.50 +/- 0.14 mm in length and 0.72 +/- 0.08 mm in width; mean +/- standard deviation of 20 samples) in mountainous crabs, Potamiscus mieni. Adult worms were successfully obtained by intraperitoneal injection with those(More)
The genetic differences among 3 closely related species, Paragonimus ohirai, Paragonimus iloktsuenensis and Paragonimus sadoensis, were assessed by electrophoretic analysis of allozymes. Fifteen enzymes were analyzed using starch gel electrophoresis. Seven of 18 loci were polymorphic. The most variable population (POs) was polymorphic at 6 of the loci (P =(More)
To assess the species of human paragonimiasis in Lao People's Democratic Republic, 6 ovum samples from 6 native confirmed paragonimiasis patients were examined with polymerase chain reaction (PCR) amplifying the internal transcribed spacer 2 (ITS2). The PCR products were sequenced, and a homology search was performed using the GenBank. All 6 sequences were(More)