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Establishment of a rapid method for detection of peste des petits ruminants virus by a reverse transcription loop-mediated isothermal amplification.
A one-step reverse transcription loop-mediated isothermal amplification(RT-LAMP) assay was developed for rapid and sensitive detection of peste des petits ruminants virus(PPRV).A set of four primersExpand
Establishment and evaluation of real-time PCR for West Nile virus detection.
A rapid Real-time polymerase chain reaction (RT-PCR) for West nile virus(WNV) detection was established. Primers were designed according to Capsid protein gene by Primer Premier5.0. In response, aExpand
Cloning and analysis of VP2 gene of canine parvovirus isolated from Beijing
The VP2 gene of canine parvovirus(CPV),isolated from Changping district of Beijing,were amplified and cloned by PCR.Using biological information software,phylogenetic tree was constructed based onExpand
Establishment and evaluation of real-time PCR for canine parvovirus detection.
A rapid real-time polymerase chain reaction(RT-PCR) for detecting of canine parvovirus(CPV) was established.Primers were designed according to VP2 protein gene by Primer Premier 5.0.In response,aExpand
Establishment and evaluation of loop-mediated isothermal amplification for detecting of porcine circovirus type 2
A loop-mediated isothermal amplification(LAMP) method was developed for the detection of porcine circovirus type 2(PCV2).A set of four primers,two outer and two inner primers,was designed based onExpand
Immunohistochemical detection of E7 human papillomavirus protein in pre‑malignant and malignant lesions of the uterine cervix
Human papillomavirus (HPV) E7 protein expression is caused by HPV viral DNA integration into human cellular DNA, and is a prerequisite for the development and progression of cervical cancer. TheExpand
Study on textures of the precision copper shaped charge material with high properties
Texture measurements and analyses of two kinds of copper shaped charge liners have been conducted by X-ray diffraction. The effects of different processings on microstructures and textures of shapedExpand
The Secondary Ring-Shaped Pipe Network Optimization Design of a District Cooling Project in Chongqing
In the paper, through a District cooling project in Chongqing, optimized design and evaluation methods of secondary loop network system are concluded, by optimizing the design of the secondaryExpand
A New Flemingia philippinensis Cultivar ‘Qianjinba 3’
‘Qianjinba 3’is a new high-yielding Flemingia philippinensis cultivar,which is derived from the excellent seedling stock plant. It has many excellent characteristics,such as its root weights 36.9 gExpand