Sherri Matis

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Detection of RNA polymerase II promoters and polyadenylation sites helps to locate gene boundaries and can enhance accurate gene recognition and modeling in genomic DNA sequence. We describe a system which can be used to detect polyadenylation sites and thus delineate the 3' boundary of a gene, and discuss improvements to a system first described in Matis(More)
To determine the role of natural killer (NK) cells in the regulation of human erythropoiesis, we studied the effects of NK-enriched cell populations on the in vitro proliferation of erythroid stem cells at three different levels of maturation (day 14 blood BFU-E, day 5-6 marrow CFU-E, and day 10-12 marrow BFU-E). NK cells were enriched from blood by Percoll(More)
Early/memory and plasma B-cell lines and fibroblasts were analyzed for their ability to use a 5' proximal (variant) versus a 3' distal (constant) poly(A) site, in the absence of a competing splice, from a set of related constructs. The proximal:distal poly(A) site use (P:D ratio) of the resulting cytoplasmic poly(A)+ mRNA is a measure of poly(A) site(More)
We found that the sequences downstream of the Ig gamma 2b secretory-specific (sec) poly(A) site play an important role in the preferential production of sec Ig mRNA during plasma B cell development. The Ig gamma 2b mRNA production in a deletion mutant (delta-Kpn) lacking the Ig sec poly(A) site and downstream consensus element (dsc) has been previously(More)
With the aim of evaluating the usefulness of an in vitro system for assessing the potential hepatotoxicity of compounds, the paper describes several methods of obtaining mathematical models for the prediction of compound-induced toxicity in vivo. These models are based on data derived from treating rat primary hepatocytes with various compounds, and(More)
Liver transplantation k a well-established therapeutic option forpatients with end-stage liver dkease. However, up to 20% of transplanted livers fail to have adequate function initially, and at least harf of those will eventually fail. Accurate, early prediction of outcome may ameliorate thk situation by encouraging retransplantation before the patient’s(More)
Itlcntifyiiig gciics witliiii largc regioiis of uricliaractcrizctl DN.4 is a difficult uiiclertakirig x id is curreiitly tlic focus of iiiii~iy rescarcli efforts. We describe a gc'iie localizatioii a i i d iriotlelirig systern. called GRXIL. GR.4IL is a multiple seiisor-iieiird rictwork Ix~sctl s p terii. It localizes genes iii moiiyirious DNX scqucricc by(More)
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