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A quantitative method based on radioimmunoassay for the determination of an endothelin receptor antagonist (C(31)H(33)NO(7), I) has been developed and validated. The immunogen was prepared by coupling I to the bovine serum albumin via the N-hydroxysuccinimide ester of I from which the radioligand was also prepared by the reaction with [125I]-iodotyrosine.(More)
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