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Given a P 1 conforming or nonconforming Galerkin finite element method (GFEM) solution p h , which approximates the exact solution p of the diffusion-reaction equation −∇ · K∇p + αp = f with full tensor variable coefficient K, we evaluate the approximate flux u h to the exact flux u = −K∇p by a simple but physically intuitive formula over each finite(More)
Penicillium marneffei is an opportunistic fungal pathogen of humans, causing respiratory, skin, and systemic mycosis in south-east Asia. Here we describe the transformation of P. marneffei with Agrobacterium tumefaciens, and the optimization of the transformation procedure. Transformations in different combinations between A. tumefaciens stains (LBA4404 and(More)
Given the anisotropic Poisson equation −∇ · K∇p = f , one can convert it into a system of two first order PDEs: the Darcy law for the flux u = −K∇p and conservation of mass ∇ · u = f. A very natural mixed finite volume method for this system is to seek the pressure in the nonconforming P1 space and the Darcy velocity in the lowest order Raviart-Thomas(More)
Penicillium marneffei is an important opportunistic dimorphic fungal pathogen that can cause fatal systemic mycosis in AIDS patients. To find new ways of overcoming infection, candidate virulence associated genes and virulence mechanisms are under intensive investigation. In the present study, we have examined the function of a novel P. marneffei histidine(More)
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