Shenghao Liu

Pengying Zhang6
Bailin Cong6
Kaoshan Chen4
Jing Wang3
6Pengying Zhang
6Bailin Cong
4Kaoshan Chen
3Jing Wang
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We have developed a system for the induction of marker-free mutation of Bacillus subtilis. The system features both the advantages of the use of antibiotic-resistance markers for mutant selection, and the ability to efficiently remove the markers, leaving unmarked mutations in the genome. It utilizes both a selective marker cassette and a counter-selective(More)
Genome-wide characterization of the Pohlia nutans transcriptome is essential for clarifying the role of stress-relevant genes in Antarctic moss adapting to the extreme polar environment. High-throughput Illumina sequencing was used to analyze the gene expression profile of P. nutans after cold treatment. A total of 93,488 unigenes, with an average length of(More)
Antarctic ice alga Chlamydomonas sp. ICE-L can endure extreme low temperature and high salinity stress under freezing conditions. To elucidate the molecular acclimation mechanisms using gene expression analysis, the expression stabilities of ten housekeeping genes of Chlamydomonas sp. ICE-L during freezing stress were analyzed. Some discrepancies were(More)
Pancreatic islet β-cells produce large amounts of γ-aminobutyric acid (GABA), which is co-released with insulin. GABA inhibits glucagon secretion by hyperpolarizing α-cells via type-A GABA receptors (GABA(A)Rs). We and others recently reported that islet β-cells also express GABA(A)Rs and that activation of GABA(A)Rs increases insulin release. Here we(More)
Under stress conditions, the mitochondrial respiratory chain generates reactive oxygen species that cause oxidative damage to lipids, proteins and nucleic acids. Mitochondrial chaperones are essential for repairing denatured proteins in plants confronted with diverse stresses. However, their functions have not been well characterized in lower plants such as(More)
Plant leucine-rich repeats receptor-like kinases (LRR-RLKs) play key roles in plant growth, development, and responses to environmental stresses. However, the functions of LRR-RLKs in bryophytes are still not well documented. Here, a putative LRR-RLK gene, PnLRR-RLK, was cloned and characterized from the Antarctic moss Pohlia nutans. Phylogenetic analysis(More)
—This paper describes a fully differential inductor-less temperature independent laser driver for a Mach-Zehnder modulator (MZM). The laser driver in this work exhibits ro-bustness in bandwidth and gain across the temperature range 27 o C to 135 o C. This driver (fabricated using a 130nm CMOS process) can achieve a differential output voltage swing of up to(More)
Plant receptor-like kinases (RLKs) are a group of conserved signaling components that regulate developmental programs and responses to biotic and abiotic stresses. However, only limited information is available on the functions of RLKs in bryophytes. Here, we described the isolation and functional characterization of a RLK gene (PnRLK-1) from the Antarctic(More)
Glutathione reductase (GR) is a flavoprotein oxidoreductase and plays an important role in response to oxidative stresses in plants. A cDNA-encoding cytosolic GR [GenBank accession number GACA01029426, designated as Pohlia nutans glutathione reductase gene (PnGR)] was successfully cloned from Antarctic moss P. nutans. The full-length PnGR cDNA has 1,654 bp(More)
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