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Is the mechanical unraveling of protein domains by atomic force microscopy (AFM) just a technological feat or a true measurement of their unfolding? By engineering a protein made of tandem repeats of identical Ig modules, we were able to get explicit AFM data on the unfolding rate of a single protein domain that can be accurately extrapolated to zero force.(More)
Escherichia coli is routinely used for the production of recombinant proteins both for research purposes and for commercial applications. Despite the extensive amount of knowledge gathered about the synthesis and accumulation of heterologous proteins and the well established principals affecting the production of secondary metabolites, little attention has(More)
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