Shelby Stamper

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Sarcocystis sp. sporocysts isolated from eight feral opossums (Didelphis virginiana) were pooled and fed to 18 commercially reared budgerigars (Melopsittacus undulatus), 14 wild-caught sparrows (Passer domesticus), one wild-caught slate-colored Junco (Junco hyemalis) and five weanling horses (Equus caballus). All budgerigars died within 5 weeks post(More)
Retroviruses encode a protease which needs to be active for the production of infectious virions. A disabling mutation in the protease results in the production of non-infectious virus particles and examination of proteins from these mutant virions reveals unprocessed Gag and Gag-Pol precursor proteins, the substrates of the viral protease. Each amino acid(More)
We have previously identified two large open reading frames, designated ORF13 and ORF25, in the Texas male-sterile cytoplasm (cms-T) of maize mitochondrial DNA (mtDNA). ORF13 is a single copy gene of chimeric origin that is uniquely transcribed and translated in the mitochondria of cms-T maize, where it produces a polypeptide of approximately 13,000 Mr. The(More)
Neospora hughesi is a recently identified cause of equine protozoal myeloencephalitis. However, the significance of this parasite is poorly understood. An enzyme-linked immunosorbent assay (ELISA) with a recombinant form of the N. hughesi 29-kDa surface antigen (rNhSAG1) was developed for serodiagnosis of equine N. hughesi infections. Parallel ELISA(More)
Sarcocystis neurona is an apicomplexan that causes equine protozoal myeloencephalitis (EPM) in North and South America. Horses appear to be an aberrant host, because the merozoites continually divide in the central nervous system, without encysting. The natural host species has not previously been identified. The small subunit ribosomal RNA (SSURNA) gene of(More)
Fecal samples were examined between 17 September and 1 November 1993 from 1765 cattle (one bull, 533 cows, 474 heifers, 22 steers, and 735 calves) on pasture on 15 farms in 11 counties in Kentucky for eggs or larvae of internal parasites. All of the cattle were beef-type except for 22 which were dairy-type. In fecal samples from the bull, cows, heifers,(More)
OBJECTIVE To determine seroprevalence of antibodies to Sarcocystis neurona in neurologically normal horses residing in 4 regions of Oregon and to describe the effects of age, gender, breed, and housing on seroprevalence within each region. DESIGN Prevalence survey. SAMPLE POPULATION Serum samples from 334 horses systematically selected by practicing(More)
OBJECTIVE To determine the seroprevalence of serum antibodies to Sarcocystis neurona in horses residing in Ohio. DESIGN Prevalence survey. SAMPLE POPULATION Serum from samples from 1,056 horses. Serum was collected on every 36th sample submitted to the Ohio State Diagnostic Laboratory for testing for equine infectious anemia. PROCEDURE Serum was(More)
Sarcocystis neurona is a coccidial parasite that causes a neurologic disease of horses in North and South America. The natural host species are not known and classification is based on ultrastructural analysis. The small subunit ribosomal RNA (SSURNA) gene of S. neurona was amplified using polymerase chain reaction techniques and sequenced by Sanger(More)
OBJECTIVE To determine seroprevalence of Sarcocystis neurona-specific antibodies in a population of horses residing in Chester County, Pa. DESIGN Prevalence survey. SAMPLE POPULATION 117 serum samples from selected members of a population of 580 Thoroughbred horses. PROCEDURE Serum was analyzed for antibodies to Sarcocystic neurona, using a western(More)