Sheilak West

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A new potato tuber lipoxygenase full-length cDNA sequence (lox1:St:2) has been isolated from potato tubers and used to express in Escherichia coli and characterize a novel recombinant lipoxygenase (potato 13/9-lipoxygenase). Like most plant lipoxygenases it produced carbonyl compounds from linoleate (the preferred substrate) and was purified in the Fe(II)(More)
The two major isoforms of lipoxygenase (LOX-2 and LOX-3) from pea (Pisum sativum L. cv. Birte) seeds have been cloned and expressed from full-length cDNAs as soluble, active, non-fusion proteins in Escherichia coli. A comparison of both isoforms purified to apparent homogeneity from E. coli and pea seeds has confirmed the authenticity of the recombinant(More)
In the developing world, ocular trauma is an important cause of monocular blindness. However, little is known about the epidemiology of eye injuries in rural Africa. This study presents five- year data on hospitalized ocular injuries in a rural region in Tanzania. Data were collected from Mvumi Hospital, a tertiary hospital serving rural Dodoma, and the(More)
AIMS/BACKGROUND This investigation determined eye care utilisation patterns in a rural county in Ireland. Population based estimates of visual impairment and glaucoma were available, so the two studies will optimise planning for eye care services for the county. METHODS Roscommon has a population of 55,000 served by one ophthalmologist and two(More)
A number of products including apocarotenal, epoxycarotenal, apocarotenone, and epoxycarotenone generated by lipoxygenase (LOX) catalyzed co-oxidation of beta-carotene have been tentatively identified through the use of GC/MS and HPLC combined with photodiode array detection. Because of the large number of high molecular weight products detected and their(More)
BACKGROUND Psychiatric disorders are common in chronic disease states; intestinal transplantation recipients may therefore be at high risk for psychiatric disorder (PD). We sought to investigate the frequency and type of PD in our cohort of patients undergoing transplantation between 2007 and 2012. RESULTS The notes of 25 patients who had undergone(More)
PURPOSE To determine if there is an association between malnutrition and clinical trachoma among children in a trachoma endemic region of Tanzania. METHODS 189 children age one to five were examined by a trained eye nurse for the presence of active trachoma, using the WHO simplified grading scheme. One examiner also measured the arm circumference of the(More)
INTRODUCTION Intestinal transplantation (IT) is considered for patients with irreversible intestinal failure who develop life-threatening complications of parenteral nutrition or have extensive intra-abdominal disease requiring evisceration. Developing indications may include quality of life (QOL) considerations and therefore assessment of QOL and(More)
PURPOSE To determine which of two village-based strategies was more effective at recruiting residents for a trachoma mass treatment campaign. METHODS The two strategies were to use either village government personnel to recruit residents for treatment, or to solicit interested community volunteers to recruit residents. Three were villages assigned to each(More)
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