Sheau-Fang Hwang

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This study investigated how the timing of application of the biofungicide Serenade (Bacillus subtilis QST713) or it components (product filtrate and bacterial cell suspension) influenced infection of canola by Plasmodiophora brassicae under controlled conditions. The biofungicide and its components were applied as a soil drench at 5% concentration (vol/vol(More)
UNLABELLED Plasmodiophora brassicae causes clubroot disease in cruciferous plants, and is an emerging threat to Canadian canola (Brassica napus) production. This review focuses on recent studies into the pathogenic diversity of P. brassicae populations, mechanisms of pathogenesis and resistance, and the development of diagnostic tests for pathogen detection(More)
Leptosphaeria maculans is a fungal pathogen causing blackleg in canola. Its virulence has been attributed, among other factors, to the activity of hydrolytic cell wall degrading enzymes (CWDEs). Studies on the pathogenicity function of CWDEs in plant pathogenic fungi have been difficult due to gene redundancy. In microorganisms many CWDE genes are repressed(More)
Clubroot, caused by Plasmodiophora brassicae, is one of the most serious diseases of cultivated cruciferous crops in the world. However, the basis for pathogenicity in P. brassicae is not well understood. In this study, a serine protease gene (PRO1) was cloned from P. brassicae and its molecular characteristics were investigated. Southern analysis and(More)
Clubroot, caused by Plasmodiophora brassicae, is an important soilborne disease of canola (Brassica napus) in Alberta, Canada. Genetic resistance is the most effective clubroot management tool, and resistant cultivars are grown extensively in affected regions. In 2013, relatively severe symptoms of clubroot were observed in some fields of resistant canola.(More)
The potential for widespreadand severe infection makes ascochyta blight,seedling blight, and root rots major hindrancesto pea production in Alberta, Canada. Over 300bacterial strains were isolated from pea seedand soil samples taken from pea fields. Thesestrains were investigated for their biologicalcontrol potential against four fungal pathogens(Pythium(More)
The disease cycle of Plasmodiophora brassicae consists of a primary phase in root hairs followed by a secondary phase in the root cortex and adjacent tissues. However, the role of root hair infection in subsequent cortical infection and development of P. brassicae is not well understood. To examine the role of the primary and secondary stages separately,(More)
To improve the efficiency of gene disruption in Stagonospora nodorum, the putative KU70 gene encoding the Ku70 protein involved in the nonhomologous end-joining double DNA break repair pathway was identified and deleted. The KU70 disruption strain showed no apparent defect in vegetable growth, conidiation and pathogenicity on wheat and barley compared with(More)
Clubroot, caused by Plasmodiophora brassicae, has two infection stages (primary and secondary). Although primary infection occurs in many plant species, secondary infection only continues to completion in susceptible hosts. As part of a larger study of clubroot pathogenesis, secondary zoospores collected from infected root hairs of canola and ryegrass were(More)