Learn More
RAPD assays were performed, using 34 arbitrary decamer oligonucleotide primers and six combinations of two primers, to detect inherent variations and genetic relationships among 12 Indian and 11 exotic B. juncea genotypes. Of 595 amplification products identified, 500 of them were polymorphic across all genotypes. A low level of genetic variability was(More)
Sclerotinia sclerotiorum is a major pathogen of many crops, including oilseed rape (Brassica napus), and there is keen interest worldwide to identify Brassica genotypes with resistance to this pathogen. However, field testing to identify resistance in B. napus germplasm is expensive, time-consuming and at times unreliable due to variability in field(More)
Male sterility conferred by ogu cytoplasm of Raphanus sativus has been transferred to Brassica juncea cv ‘RLM 198’ from male-sterile B. napus through repeated backcrossing and selection. The male-sterile B. juncea is, however, highly chlorotic and late. It has low female (seed) fertility and small contorted pods. To rectify these defects, protoplasts of the(More)
Association mapping of the seed-coat colour with amplified fragment length polymorphism (AFLP) markers was carried out in 39 Brassica juncea lines. The lines had genetically diverse parentages and varied for seed-coat colour and other morphological characters. Eleven AFLP primer combinations were used to screen the 39 B. juncea lines, and a total of 335(More)
The tournefortii cytoplasmic male-sterility system is being used as a method of pollination control to develop hybrids in Brassica napus. Genetic analyses have indicated that two dominant genes, one major (Rft1) and another minor (Rft2), were required to achieve complete fertility restoration. Though the major gene (Rft1) can cause complete fertility(More)
An intergeneric hybrid between a wild species, Erucastrum canariense (2n = 18; ECEC), and a cultivated oilseed brassica species, Brassica rapa (2n = 20; AA), was synthesized through ovary culture in White's basal medium supplemented with casein hydrolysate. Morphological, cytological and DNA-based analysis helped to establish the hybrid nature of the(More)
The cytoplasmic substitution lines of Brassica juncea (L.) Coss were evaluated for their field resistance to Alternaria blight (Alternaria brassicae). The euplasmic B. juncea cv. ‘RLM 198’ had a mesothetic reaction while alloplasmic B. juncea lines with cytoplasms of B. campestris, B. chinensis, and B. japonica were highly susceptible. B. nigra cytoplasm(More)
In the present study fifty genotypes of Brassica juncea were evaluated for heat stress tolerance in terms of biochemical components, in four day old seedlings. Heat shock was given at 45 degrees C for 4.5 hr and thereafter survival percentage, electrolyte leakage and chlorophyll content were estimated. Tolerant genotypes (10) registered survival greater(More)
Two wide hybrids, Diplotaxis erucoides (2n = 14) × Brassica rapa (2n = 20) and B. maurorum (2n = 20) × B. rapa, were developed using the sequential ovary–ovule culture. Reciprocal crosses failed, possibly as a consequence of strong unilateral incompatibility. The F 1 hybrids in each combination were completely male sterile and morphologically intermediate(More)
Biochemical studies were conducted on some male sterile and their fertile F1 analogues in Indian mustard. The variation in pH activity during microsporogenesis was normal, except in MS-3. Male sterile anthers had deficient sugar metabolism. Cytochemical analysis of sporogenous and tapetal tissue suggested an effect of sterility elements on the anabolic and(More)