Sharyn K. Freyermuth

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Presented here is the complete genome sequence of Thiomicrospira crunogena XCL-2, representative of ubiquitous chemolithoautotrophic sulfur-oxidizing bacteria isolated from deep-sea hydrothermal vents. This gammaproteobacterium has a single chromosome (2,427,734 base pairs), and its genome illustrates many of the adaptations that have enabled it to thrive(More)
Mutation in Eu3 eliminates activity of both soybean ureases, the embryo-specific (encoded by Eu1) and the tissue-ubiquitous (encoded by Eu4). eu3-e1 is a completely recessive null allele. Eu3-e3 is a semi-dominant specifying 0.1% wild-type urease activity in the homozygous state and 5-10% as a heterozygote (Meyer-Bothling et al. 1987). Antibodies to plant(More)
In order to evaluate whether Co can functionally replace Ni in urease, as suggested by Watanabe et al. (Soil Sci. Plant Nutr. (1994) 40, 545–548), zucchini and soybean plants were grown in purified nutrient solutions containing nitrate and ammonium as sole N source supplemented either with 0.05 mmol m-3 Ni or Co or with no supplementation (control). In(More)
Arginase (L-Arg amidinohydrolase, EC 3.5.3.1) catalyzes the hydrolysis of L-Arg to urea and L-Orn. Orn is a precursor of Pro and polyamines and urea N is recycled by urease-catalyzed hydrolysis to ammonia (Polacco and Holland, 1993). Many plant species store much seed protein N in Arg (VanEtten et al., 1963), significant quantities of which are released and(More)
1 Department of Biology, University of St. Thomas, St. Paul, Minnesota, United States of America, 2 Department of Biological Sciences, St. Cloud State University, St. Cloud, Minnesota, United States of America, 3 Department of Biology, Hiram College, Hiram, Ohio, United States of America, 4 Biochemistry Department, University of MissouriColumbia, Columbia,(More)
Embedded assessments, tasks within the context of instruction, can serve as important tools for improving teaching and learning. In this article, we report on the assessment design principles and a nine-step development model that guided the construction of innovative embedded assessments. Using multiple sources of data-expert reviews, a conceptual test(More)
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