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The method of surface-enhanced Raman microspectroscopy was developed for direct detection of membrane-bound enzymes in cells. Cells were cultured, fixed, and incubated with specific primary… (More)
A method using confocal Raman microspectroscopy for the detection of cellular proteins in single intact cells was developed. Two approaches were used to improve the detection of these cellular… (More)
Raman microspectroscopy was used to characterize normal and malignant hepatocytes in both cultured cells and human liver tissues. Consistent spectral changes were observed, including intensity… (More)
Raman microspectroscopy is presented as a promising technique for the in situ characterization of intracellular cholesterol crystals. Crystal characterization is the first step in investigating the… (More)
A diphenyloctatetraene (DPOT) doped bibenzyl crystal is a model system for studying the spectroscopic properties of polyenes because the well‐defined local environment of the DPOT eliminates most of… (More)