Shaoning Chen

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MicroRNAs (miRNAs) are RNA molecules consisting of 20-24 nucleotides that play important roles in regulating plant's gene expression for growth and development, cell viability and stress responses. Viral infection often has a noticeable influence on host gene expression, which may result in a range of developmental abnormalities. To investigate the(More)
MicroRNAs (miRNAs) are a newly identified class of non-coding small RNAs of about 21–24 nucleotides. They play important roles in multiple biological processes by degrading targeted mRNAs or repressing mRNA translation. To date, a total of 2,043 plant miRNAs are present in the miRNA Registry database (miRBase Release 14.0), and none for tobacco (Nicotiana(More)
Magnesium (Mg) is one of the most important nutrients, involves mainly in plant growth and development. However, the signaling pathways response to magnesium stresses (MgSs) is known little, but several studies lately may improve the research development. Several phytohormones such as abscisic acid (ABA), ethylene, auxins, and their factors were found(More)
MicroRNAs (miRNAs) are a class of small RNAs that affect the morphological and physiological development of plants. In recent years, there is accumulating evidence that miRNAs are involved in defense mechanism of host plants. Therefore, investigating the alteration of miRNAs expression profiles after virus infection will provide new insights for(More)
cDNA containing the 3′ part sequences of seven Lily symptomless virus (LSV) isolates were obtained by RT-PCR and sequenced. The sequenced region is corresponding to partial CP gene and the entire sequences of 16kDa gene located at the 3′ end of LSV genome. The sequence homologies of partial CP and 16 kDa protein genes, compared with(More)
Cucumber mosaic virus (CMV) is widely spread worldwide, causing typical systemic mosaic and other symptoms in tomato (Solanum lycopersicum). Host responses to CMV and molecular mechanisms associated with the development of disease symptoms caused by this virus in tomato are largely unexplored. To investigate plant responses activated during this(More)
A multiplex reverse-transcription polymerase chain reaction (RT-PCR) protocol was developed for simultaneous detection and discrimination of subgroups of Cucumber mosaic virus (CMV), including its satellite RNA, Tomato mosaic virus (ToMV) and Tobacco mosaic virus (TMV), using 18S rRNA as an internal control. Species- and subgroups-specific primers designed(More)
MicroRNAs (miRNAs) reported previously from Arabidopsis. thaliana and other seven plant species, by both experimental validation and prediction, were used to identify potential miRNAs in Saccharum officinarum which is an important food and bio-energy crop. A microfluidic microarray was developed for detecting plant miRNAs and used for the first experimental(More)
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