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In this study, an Escherichia coli (E. coli) based transcription translation cell-free system (TX-TL) was employed to sample various enzyme expression levels of the violacein pathway. TX-TL enables rapid modifications and prototyping of the pathway without complicated cloning cycles. The violacein metabolic pathway has been successfully reconstructed in(More)
In this study, an Escherichia coli (E. coli) based transcription translation cell-free system (TX-TL) was employed to sample various enzyme expression levels of the violacein pathway. The pathway was successfully reconstructed in TX-TL. Its variation produced different metabolites as evident from the extracts' assorted colors. Analysis of the violacein(More)
In wireless navigation system worked on medium-short wave, skywave interference is a serious problem which influences the work region and orientation precision. Based on the model analysis of skywave interference, the method of using multi-user detection technique to eliminate sky wave interference is proposed. Estimate and rebuild the strong skywave(More)
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