Shanti Sarup

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Bovine tropical theileriosis caused byTheileria annulata is an economically important disease of cattle in India. The disease has assumed paramount importance with the intensification of cross-breeding programmes aimed at enhancing milk production in the country. To control this disease, a cell culture vaccine was developed in this department by continuous(More)
Bovine babesiosis, caused byBabesia bigemina, is an important tick-transmitted haemoprotozoan disease in the tropics. This study evaluated the immunoprotective efficacy ofin vitro producedB. bigemina exoantigens in bovine calves. The calves inoculated withB. bigemina exoantigens did not show any clinical, parasitological or hypersensitivity reactions after(More)
An antigen was prepared from the spleen of an infected bovine calf and was used in the tube agglutination test. Cases having rare and very low parasitaemia as well as the carrier cases were detected by this procedure and no non-specific reaction was observed with other haemoprotozoan infections. The maximum titre obtained with theAnaplasma positive sera was(More)
Efficacy of medium RPMI-1640 (supplied by Gibco USA, Centron and Hi-media) supplemented with horse, donkey, sheep and goat sera was evaluated for in vitro propagation of Theileria annulata (Hisar) infected bovine mononuclear cells. The results were compared with the growth rate in RPMI-1640 supplemented with foetal bovine serum (Gibco). RPMI-1640 (Gibco)(More)
An intradermal skin test, to demonstrate a delayed cutaneous hypersensitivity reaction inBabesia equi infection in donkeys, was developed. A skin reaction toB. equi antigen was elicited in vaccinated, infected and carrier intact and splenectomised donkeys. The histopathological examination of the skin biopsy revealed infiltration of mononuclear cells and(More)
The cell-mediated immune response (CMIR) was demonstrated during experimental Anaplasma marginale infection in cattle, in vivo by the intradermic skin test and in vitro by the leucocyte migration inhibition test (LMIT). The skin test showed a delayed hypersensitivity reaction which reached its maximum after 48-72 hours. Histopathological examination of the(More)
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