Shailesh S. Deshpande

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Increased steady shear stress stimulates nitric oxide synthase (eNOS) in part by Akt-dependent phosphorylation. Arteries in vivo are exposed to pulse perfusion (PP) combining phasic shear with stretch. In compliant vessels, enhancing PP lowers vascular tone by stimulating eNOS; whereas in aged, stiff arteries, flow-mediated dilation declines and PP is a(More)
Production of reactive oxygen species (ROS) by ischemic tissue after ischemia-reperfusion (I/RP) is an important factor that contributes to tissue injury. The small GTPase Rac1 mediates the oxidative burst, and ROS act on signaling pathways involved in expression of inflammatory genes. Because there is evidence implicating monocytes in the pathogenesis of(More)
The effects of turmeric feeding before and after benzo(a)pyrene [B(a)P] exposure on the levels of B(a)P-derived DNA adducts were studied in tissues of Swiss mice employing (32)P-postlabelling analysis. A reduction in the levels of B(a)P-derived DNA adducts in liver, lung, and forestomach was observed in animals pre-treated with 0.2 or 1% turmeric diet and(More)
OBJECTIVE Oxidative stress has been implicated in cellular senescence and vascular aging. We determined the role and mechanism of the small GTPase rac1 in vascular endothelial cell senescence. METHODS AND RESULTS Adenoviral-mediated expression of the constitutively active allele of rac1 (rac1V12) in human umbilical vein endothelial cells resulted in(More)
In nonexcitable cells, stimulation by high agonist concentrations typically produces a biphasic increase in cytosolic Ca ([Ca]i). This response is characterized by a transient initial increase because of intracellular Ca release followed by a sustained elevation which varies in amplitude depending on the nature of the stimulus. In contrast, low-level(More)
In this paper, we suggest optimal flat fields for calibration of hyperspectral data (EO1) — predominantly of urban nature. We compare the suitability of various flat field candidates by performing vegetation, soil, and impervious surface (VIS) classification. Flat Field methods provide 90% average overall accuracy (with best 100% and 77% worst(More)
In nonexcitable cells, stimulation by high agonist concentrations typically produces a biphasic increase in cytosolic Ca ([Ca]i). This response is characterized by a transient initial increase because of intracellular Ca release followed by a sustained elevation which varies in amplitude depending on the nature of the stimulus. In contrast, low-level(More)
Objective of this research is to compare performance of the Internal Areal Relative Reflectance (IAR), and Second Simulation of a Satellite Signal in the Solar Spectrum (6SV) surface calibration methods for discriminating impervious surfaces in urban settings. We used EO1-Hyperion image of Pune city recorded on April 2013. We converted the radiance values(More)
BACKGROUND The release of Weibel-Palade Bodies (WPB) is a form of endothelial cell activation. But the signal transduction pathway leading to WPB release is not yet defined. We hypothesized that small G-protein rac1 and reactive oxygen species (ROS) mediate the ligand induced release of Weibel-Palade Bodies. METHODS We tested this hypothesis by using(More)