Seung Pyo Gong

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OBJECTIVE To suggest an alternative strategy for deriving histocompatible stems cells without undertaking genetic manipulation. DESIGN Prospective approach using an animal model. SETTING Stem cell and bioevaluation laboratory, Seoul National University. ANIMAL(S) F1 (C57BL6 X DBA2) and outbred (ICR) mice. INTERVENTION(S) Ovarian stroma cells of less(More)
The aim of this study was to establish a basic manipulation protocol of preantral follicles for deriving developmentally competent oocytes. Primary, early and late secondary follicles retrieved from the ovaries of 14-day-old F1 (C57BL/6 x DBA2) female mice mechanically or enzymatically were cultured singly and in vitro growth of the follicles and maturation(More)
OBJECTIVE To optimize embryo culture system by evaluating lighting effect during in vitro manipulation. DESIGN Randomized prospective study using an animal model. SETTING Gamete and Stem Cell Biotechnology Laboratory at Seoul National University in Korea. ANIMAL(S) Twelve- to 15-week-old hamsters (Mesocricetus auratus). INTERVENTION(S) Two-cell(More)
OBJECTIVE To evaluate the effect of antioxidant combinations on human sperm motility and morphologic normality during preparation and in vitro incubation to improve sperm quality for assisted reproductive technology (ART). DESIGN Prospective study. SETTING Laboratory. PATIENT(S) Six fertile males, 21 to 25 years of age, after 3 days of sexual(More)
OBJECTIVE To determine whether the preantral follicles in adult ovaries can generate developmentally competent oocytes after in vitro culture. DESIGN Prospective, animal-model study. SETTING Gamete and Stem Cell Biotechnology Laboratory, Seoul National University, Seoul, Korea. ANIMAL(S) B6CBAF1 mice. INTERVENTION(S) Preantral follicles collected(More)
OBJECTIVE To evaluate whether autologous embryonic stem cells can be established without generating clone embryos. DESIGN Prospective model study. SETTING Gamete and stem cell biotechnology laboratory in Seoul National University, Seoul, Korea. ANIMAL(S) F1 hybrid B6D2F1 mice. INTERVENTION(S) Preantral follicles were cultured, and oocytes matured in(More)
The development of species-specific fish cell lines has become a valuable tool for biological research. In recent years, marine medaka Oryzias dancena has been recognized as a good experimental model fish but there are no reports of establishment of cell lines from this fish. In this study, two cell lines from O. dancena blastula embryos were established(More)
To study the genomic plasticity of somatic cells without ectopic genetic manipulation, we cultured mouse fibroblasts with ovarian cells, embryonic fibroblasts of different strains, and parthenogenetic embryonic stem cells (ESCs). Of 41 trials, cell aggregation resembling nascent ESC colony from inner cell mass was detected in 9 cases (22%), and 6 cases(More)
Somatic cell nuclear transfer, the first established technique for producing patient-specific autologous stem cells, inevitably requires the sacrifice of viable embryos. To circumvent the serious ethical issues associated with this use of embryos, researchers have developed several alternative methods for the production of histocompatible stem cells. In our(More)
This study was conducted to establish the efficient condition for stable derivation of heart-derived cell culture in Siberian sturgeon (Acipenser baerii). Three factors including isolation methods, cell densities in initial seeding, and basal media were evaluated for the derivation of heart-derived cell culture. As the results, enzymatic isolation was more(More)