Seung-Jung Ha

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OBJECTIVE To study the influence of sugars and establish a serum-free freezing method for the cryopreservation of spermatogonial stem cells (SSCs). DESIGN Animal study. SETTING University laboratory. ANIMAL(S) C57BL/6-TgEGFP, C57BL/6 mice. INTERVENTION(S) Germ cells enriched from testis cells were frozen using standard freezing medium containing(More)
Spermatogonial stem cells (SSCs) are germline stem cells that serve as the foundation of spermatogenesis to maintain fertility throughout a male's lifetime. To treat male infertility using stem cell banking systems and transplantation, it is important to be able to preserve SSCs for long periods of time. Therefore, this study was conducted to develop an(More)
Primordial germ cells (PGCs) as well as fetal germ line stem cells (GSCs) are pluripotent cells. Their differentiation potential is similar to that of embryonic stem cells (ESCs), suggesting that germ line lineage may retain the potential to form pluripotent cells. Here we report successful establishment of multipotent germ line stem cells (mGSCs) from(More)
Spermatogonial stem cells (SSCs) have unique abilities to self-renew and differentiate into committed cells that can finally produce sperm. Glial cell line-derived neurotrophic factor (GDNF) has been reported as a crucial factor for SSC self-renewal. However, the intracellular mechanisms of SSC self-renewal remain unclear. To investigate these mechanisms,(More)
Spermatogonial stem cells (SSCs) are adult male germ cells that develop after birth. Throughout the lifetime of an organism, SSCs sustain spermatogenesis through self-renewal and produce daughter cells that differentiate into spermatozoa. Several studies have demonstrated that SSCs can acquire pluripotency under appropriate culture conditions, thus becoming(More)
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