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Helicase-DNA polymerase interaction is critical to initiate leading-strand DNA synthesis
Interactions between gene 4 helicase and gene 5 DNA polymerase (gp5) are crucial for leading-strand DNA synthesis mediated by the replisome of bacteriophage T7. Interactions between the two proteinsExpand
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  • Open Access
Direct role for the RNA polymerase domain of T7 primase in primer delivery
Gene 4 protein (gp4) encoded by bacteriophage T7 contains a C-terminal helicase and an N-terminal primase domain. After synthesis of tetraribonucleotides, gp4 must transfer them to the polymerase forExpand
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A unique loop in T7 DNA polymerase mediates the binding of helicase-primase, DNA binding protein, and processivity factor.
Bacteriophage T7 DNA polymerase (gene 5 protein, gp5) interacts with its processivity factor, Escherichia coli thioredoxin, via a unique loop at the tip of the thumb subdomain. We find that thisExpand
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Essential Residues in the C Terminus of the Bacteriophage T7 Gene 2.5 Single-stranded DNA-binding Protein*
Gene 2.5 of bacteriophage T7 encodes a single-stranded DNA (ssDNA)-binding protein (gp2.5) that is an essential component of the phage replisome. Similar to other prokaryotic ssDNA-binding proteins,Expand
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An Interaction between DNA Polymerase and Helicase Is Essential for the High Processivity of the Bacteriophage T7 Replisome*
Background: Interactions of DNA polymerase and DNA helicase are crucial in DNA synthesis. Results: Two distinct interactions are involved in formation of the DNA polymerase/DNA helicase complex.Expand
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Dynamic Changes in Ester Formation during Chardonnay Juice Fermentations with Different Yeast Inoculation and Initial Brix Conditions
Ester formation was monitored by solid-phase microextraction (SPME) during alcoholic fermentations of Chardonnay juice. Repeated batch fermentations were conducted with two yeast inoculation levelsExpand
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Changes in Physicochemical Characteristics of Black Raspberry Wines from Different Regions During Fermentation
In this study, four Korean black raspberry wines were developed using raspberries from different regions in Korea; Gochang (G), Hoengseong (H), Jeongeup (J), Sunchang (S). Sample wines were monitoredExpand
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  • Open Access
Primer initiation and extension by T7 DNA primase
T7 DNA primase is composed of a catalytic RNA polymerase domain (RPD) and a zinc‐binding domain (ZBD) connected by an unstructured linker. The two domains are required to initiate the synthesis ofExpand
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Heterohexamer of 56- and 63-kDa Gene 4 Helicase-Primase of Bacteriophage T7 in DNA Replication*
Background: The role of the 56-kDa gene 4 helicase-primase in T7 DNA replication is unknown. Results: Individual hexamers of the 56-, 63-, or a mixture of the two have identical helicase activity. AExpand
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Gene 5.5 protein of bacteriophage T7 in complex with Escherichia coli nucleoid protein H-NS and transfer RNA masks transfer RNA priming in T7 DNA replication
DNA primases provide oligoribonucleotides for DNA polymerase to initiate lagging strand synthesis. A deficiency in the primase of bacteriophage T7 to synthesize primers can be overcome by geneticExpand
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