Sergey N. Mikhailov

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A new anticytokinin N 6 (benzyloxymethyl)adee nosine (BOMA), an antagonist of the cytokinin recepp tor CRE1/AHK4 of Arabidopsis, was found among the synthetic derivatives of N 6 adenosine. The new antii cytokinin BOMA, described by us, is highly specific: it inhibits the activation of the CRE1/AHK4 receptor but not the AHK3 receptor with a similar(More)
The use of chemically synthesized short interfering RNAs (siRNAs) is currently the method of choice to manipulate gene expression in mammalian cell culture, yet improvements of siRNA design is expectably required for successful application in vivo. Several studies have aimed at improving siRNA performance through the introduction of chemical modifications(More)
The effect of sulfated polysaccharides on the efficiency of the infection of mouse transplantable fibroblast SC-1 and NIH-3T3 cell lines by replication-competent recombinant Moloney murine leukemia virus (Mo-MuLV), which carries the eGFP gene, was investigated. It was found that sulfated polysaccharides have no cytostatic and cytotoxic effects on SC-1 and(More)
The influence of the conditions of the formation of chitosan hydrogels crosslinked with glutaraldehyde (GA) or genipin (the polysaccharide molecular weight, pH level, and concentration of the chitosan solution) on the gel time and the properties of biopolymer scaffolds for tissue engineering obtained by the freeze-drying of hydrogels was studied. The(More)
The ability of 7-methylguanine, a nucleic acid metabolite, to inhibit poly(ADP-ribose)polymerase-1 (PARP-1) and poly(ADP-ribose)polymerase-2 (PARP-2) has been identified in silico and studied experimentally. The amino group at position 2 and the methyl group at position 7 were shown to be important substituents for the efficient binding of purine(More)
187 Uridine phosphorylase (UP) (EC 2.4.2.3) belongs to nucleoside phosphorylases that play an important role in nucleoside metabolism [1]. These enzymes exhibit an elevated level of activity in tumor cells, due to which they can be used as markers of cancer diagg nosis [2]. These enzymes are used in the industry for the synthesis of drugs and nucleosides of(More)
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