Sergey M Khomutov

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The strain of Mycobacterium sp. VKM Ac-1817D forms 9α-hydroxy-androst-4-ene-3,17-dione (9-OH-AD) as a major product from sitosterol. The formation of 9-OH-AD was accompanied with its partial destruction due to residual steroid-1-dehydrogenase (St1DH) activity. The activity was found to be induced by androst-4-ene-3,17-dione (AD), while other intermediates(More)
Cyclodextrins (CDs) are widely used in pharmacology as biocompatible and nontoxic carriers of biologically active agents. The use of various drugs in the form of CD-based inclusion complexes (of the host – guest type) increases the solubility and stability of the parent drugs, facilitates delivery of the hydrophobic components to the target organ, and(More)
Two-step one-pot microbial transformation enables obtaining of valuable steroids that are difficult to produce chemically. Here we describe a method for obtaining 11α-hydroxyandrost-4-ene-3,17-dione (11α-HAD) from cheap and available natural sterols (phytosterols or cholesterol).11α-HAD is a primary adrenal steroid in mammals and also a key precursor in the(More)
A nonlinear spectrometric method for determination of the stability constant (Ks) for cyclodextrin complex with steroid was developed. The method is based on calculation of the parameters of competitive cyclodextrin complexation by simultaneous fitting of two types of curves. Those of the first type are the dependencies of absorbance of methyl orange(More)
The synthesis of 3beta-hydroxy-androsta-5,7-dien-17-one from 3beta-hydroxy-androst-5-en-17-one (dehydroepiandrosterone, DHEA) via microbial 7alpha-hydroxylation has been accomplished. At the first stage, 3beta,7alpha-dihydroxy-androst-5-en-17-one was obtained in high yield (71.2%) using a strain of Gibberella zeae VKM F-2600, which was first applied for(More)
The transformation of dehydroepiandrosterone by Spicaria fumoso-rosea VKM F-881 produced 7α- and 7β-hydroxy-dehydroepiandrosterone, 3β,7α-dihydroxy-17a-oxa-D-homo-androst-5-en-17-one, and 3β,7β-dihydroxy-17a-oxa-D-homo-androst-5-en-17-one. The yield of the main product—3β,7β-dihydroxy-17a-oxa-D-homo-androst-5-en-17-one—was 49.5–72 mol % at substrate(More)
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