Sergey A Sedov

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The key factors of enzymatic lysis of cells are the interaction between the enzyme and the cell - catalytic and non-catalytic adsorption of enzyme on cell surface. Here, the studies of lysis of intact Escherichia coli cells by chicken egg white lysozyme were performed. It was found that the ionic strength has a dual effect onto the system. On the one hand,(More)
Researchers have related the manufacturing technique of plasters and stucco in the Maya area with their period of production but not with their architectural function. In this paper, we establish a relationship between those three features (manufacturing technique, age, and architectural function) in the plasters of the Maya site of Dzibanché in southern(More)
In this paper we report the discovery of bacteriolytic activity of an immune system cytokine mediator, interleukin-2. Bacteriolytic activity of interleukin-2 was compared with a well-known bacteriolytic enzyme — chicken egg white lysozyme — by monitoring the lysis of the Gram-negative bacterium Escherichia coli, the Gram-positive coccus Micrococcus luteus,(More)
Bacteriophage enzyme preparations exolysin and endolysin were studied. Exolysin (a phage-associated enzyme) was obtained from tail fraction and endolysin from phage-free cytoplasmic fraction of disintegrated Salmonella enteritidis cells. A new method for purification of these enzymes was developed, and their molecular masses were determined. The main(More)
In the present work the studies ofbacteriolytic factors from sheep blood plasma have been performed. Three novel enzymes have been identified and characterized. Two of them have a molecular weight 15 +/- 2 kDa and able to lyse the gram-negative Escherichia coli bacteria. The third enzyme has a molecular weight 34 +/- 4 kDa and is able to lyse both(More)
(2015) A detailed paleomagnetic and rock-magnetic investigation of the Matuyama-Brunhes geomagnetic reversal recorded in the tephra-paleosol sequence of Tlaxcala (Central Mexico). A detailed paleomagnetic and rock-magnetic investigation of the Matuyama-Brunhes geomagnetic reversal recorded in the tephra-paleosol sequence of Tlaxcala (Central Mexico)(More)
In this Technical Note, the quantitative turbidimetric assay for determination of the bacteriolytic activity of enzymes with gram-negative bacteria is proposed. The reactivity of hen white-egg lysozyme toward gram-negative E. coli intact cells was studied. It was found that the highest lysis rate occurred at pH 8.9 in the system containing 0.03 M NaCl. The(More)