Sergej Kakorin

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Electrooptical and conductometrical relaxation methods have given a new insight in the molecular mechanisms of the electroporative delivery of drug-like dyes and genes (DNA) to cells and tissues. Key findings are: (1) Membrane electroporation (ME) and hence the electroporative transmembrane transport of macromolecules are facilitated by a higher curvature(More)
Membrane electroporation is the method to directly transfer bioactive substances such as drugs and genes into living cells, as well as preceding electrofusion. Although much information on the microscopic mechanism has been obtained both from experiment and simulation, the existence and nature of possible intermediates is still unclear. To elucidate(More)
Detailed kinetic data suggest that the direct transfer of plasmid DNA (YEp 351, 5.6 kbp, supercoiled, Mr approximately 3.5 x 10(6)) by membrane electroporation of yeast cells (Saccharomyces cerevisiae, strain AH 215) is mainly due to electrodiffusive processes. The rate-limiting step for the cell transformation, however, is a bimolecular DNA-binding(More)
The color change of electroporated intact immunoglobulin G receptor (Fc gammaR-) mouse B cells (line IIA1.6) after direct electroporative transfer of the dye SERVA blue G (Mr 854) into the cell interior is shown to be dominantly due to diffusion of the dye after the electric field pulse. Hence the dye transport is described by Fick's first law, where, as a(More)
The phenomenon of membrane electroporation (ME) methodologically comprises an electric technique to render lipid and lipid-protein membranes porous and permeable, transiently and reversibly, by electric voltage pulses. It is of great practical importance that the primary structural changes induced by ME, condition the electroporated membrane for a variety(More)
The ionic conductivity of lipid membrane pores has been theoretically analysed in terms of electrostatic interactions of the transported ions with the low-dielectric pore wall for a commonly encountered case of unequal concentrations of electrolyte on the two sides of curved lipid membranes. Theoretical analysis of the data on the conductivity of the(More)
The electric (linear) dichroisms observed in the membrane electroporation of salt-filled lipid bilayer vesicles (diameter O = 2 alpha = 0.32 micron; inside [NaCl] = 0.2 M) in isotonic aqueous 0.284 M sucrose-0.2 mM NaCl solution indicate orientation changes of the anisotropic light scattering centers (lipid head groups) and of the optical transition moments(More)
Isothermal titration calorimetry (ITC) is a powerful technique for investigating self-association processes of protein complexes and was expected to reveal quantitative data on peroxiredoxin oligomerization by directly measuring the thermodynamic parameters of dimer-dimer interaction. Recombinant classical 2-cysteine peroxoredoxins from Homo sapiens,(More)
Analysis of the reduced turbidity (delta T-/T0) and absorbance (delta A-/A0) relaxations of unilamellar lipid vesicles, doped with the diphenylhexatrienyl-phosphatidylcholine (beta-DPH pPC) lipids in high-voltage rectangular electrical field pulses, demonstrates that the major part of the turbidity and absorbance dichroism is caused by vesicle elongation(More)
Electric fields, similar in the order of magnitude of the natural membrane fields of cellular lipid/protein membranes, and chemical relaxation spectrometry can be used as tools to quantify the rigidifying effect of cholesterol in membranes. Small unilamellar vesicles of radius a=50+/-3 nm, prepared form phosphatidylcholine, phosphatidylserine and(More)