Sergei E Dmitriev

Learn More
Rhopalosiphum padi virus (RhPV) is an insect virus of the Dicistroviridae family. Recently, the 579-nucleotide-long 5' untranslated region (UTR) of RhPV has been shown to contain an internal ribosome entry site (IRES) that functions efficiently in mammalian, plant, and insect in vitro translation systems. Here, the mechanism of action of the RhPV IRES has(More)
A relaxed cap-dependence of translation of the mRNA-encoding mammalian heat shock protein Hsp70 may suggest that its 5'-untranslated region (UTR) possesses an internal ribosome entry site (IRES). In this study, this possibility has been tested in transfected cells using plasmids that express dicistronic mRNAs. Using a reporter gene construct, Renilla(More)
The reconstitution of translation initiation complexes from purified components is a reliable approach to determine the complete set of essential canonical initiation factors and auxiliary proteins required for the 40S ribosomal subunit to locate the initiation codon on individual mRNAs. Until now, it has been successful mostly for formation of 48S(More)
We have earlier shown that the 5′-untranslated region (5′ UTR) of the mRNA coding for activation factor of apoptotic peptidase 1 (Apaf-1) can direct translation in vivo by strictly 5′ end-dependent way even in the absence of m7G-cap. Dependence of translational efficiency on the cap availability for this mRNA turned out to be relatively low. In this study(More)
Translation initiation in eukaryotic cells is known to be a complex multistep process which involves numerous protein factors. Here we demonstrate that leaderless mRNAs with initiator Met-tRNA can bind directly to 80S mammalian ribosomes in the absence of initiation factors and that the complexes thus formed are fully competent for the subsequent steps of(More)
According to the generally accepted scanning model proposed by M. Kozak, the secondary structure of the 5′-untranslated region (5′-UTR) of eukaryotic mRNA can only inhibit the translation initiation by counteracting migration of the 40S ribosomal subunit along the mRNA polynucleotide chain. The existence of efficiently translatable mRNAs with long and(More)
Nucleotide sequence changes increasing the number of paired bases without producing stable secondary structure in the 5"-untranslated region (5"-UTR) of the β-globin mRNA had a slight effect on its translation in rabbit reticulocyte lysate at low mRNA concentration and dramatically decreased translation efficiency at a high concentration. The removal of(More)
The L1 retrotransposon codes for a unique bicistronic mRNA, which serves as a transposition intermediate and as a template for the synthesis of two proteins. According to preliminary data, the translation of both cistrons is initiated by a noncanonical mechanism. The L1 mRNA was translated in rabbit reticulocyte lysate (RRL), a standard system widely used(More)
Three—classical cap-dependent, shunting, and IRES-dependent—major mechanisms are now known for mRNA association with the 40S ribosome subunit in eukaryotes [1]. In the IRES-dependent mechanism, the 40S subunit binds to a specific internal ribosome entry site (IRES) in the 5'-untranslated region of mRNA, rather than interacting with its 5' end. A classical(More)
The long 5′-untranslated region (5′-UTR) of the human L1 retrotransposon contains a unique internal promoter, allowing new L1 copies to be less dependent on the integration site at the transcriptional level. The mechanism of action of this promoter still remains unclear; however, some early studies have build up an opinion that the first 5′-UTR segment of(More)