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Males of Drosophila melanogaster lacking the Y chromosome-linked crystal locus show multiple meiotic alterations including chromosome disorganization and prominent crystal formation in primary spermatocytes. These alterations are due to the derepression of the X chromosome-linked Stellate sequences. To understand how the derepression of the Stellate(More)
The availability of genomic clones representative of the T-cell receptor constant gamma (TRGC) ovine genes enabled us to demonstrate, by fluorescent in situ hybridization (FISH) on cattle and sheep metaphases, the presence of two T-cell receptor gamma (TRG1@ and TRG2@) paralogous loci separated by at least five chromosomal bands on chromosome 4. Only TRG1@(More)
gammadelta T cells commonly account for 0.5%-5% of human (gammadelta low species) circulating T cells, whereas they are very common in chickens, and they may account for >70% of peripheral cells in ruminants (gammadelta high species). We have previously reported the ovine TRG2@ locus structure, the first complete physical map of any ruminant animal TCR(More)
A series of genomic clones derived from a sheep library were used to determine the germline configuration and the exon-intron organization of TRGC2, TRGC3, and TRGC4 genes. Based on the outcomes of molecular analysis, we compared and aligned the genomic sequences with the known complete cDNA sequences of sheep and deduced the exon-intron organization of(More)
In vitro fertilization (IVF) has had poor success in the horse, a situation related to low rates of sperm penetration through the zona pellucida (ZP). Zona pellucida hardening (ZPH) is seen in mouse and rat oocytes cultured in serum-free medium. The hardened ZP is refractory to sperm penetration. Fetuin, a component of fetal calf serum, inhibits ZPH and(More)
In mammals, T cells develop along two discrete pathways characterized by expression of either the αβ or the γδT cell receptors. Human, mouse, and dog display a low peripheral blood γδ T cell percentage, while sheep accounts for a high proportion of γδ T lymphocytes. In all these species, the genomic organization of the T cell receptor gamma (TRG) locus is(More)
In jawed vertebrates the V-(D)-J rearrangement is the main mechanism generating limitless variations of antigen-specific receptors, immunoglobulins (IGs), and T-cell receptors (TCRs) from few genes. Once the initial diversity is established in primary lymphoid organs, further diversification occurs in IGs by somatic hypermutation, a mechanism from which(More)
In accordance with the anchorage site hypothesis, Daudi beta 2-microglobulin (-) HLA (-) human male Burkitt lymphoma cells are incapable of stably maintaining H-Y antigen on their plasma membrane; instead, they excrete it into the culture medium. The proposed testis-organizing function of Daudi H-Y antigen in solution, previously demonstrated in bovine(More)
Molecular cloning of cDNA from gamma/delta T cells has shown that in sheep, the variable domain of the delta chain is chiefly determined by the expression of the TRDV1 subgroup, apparently composed of a large number of genes. There are three other TRDV subgroups, but these include only one gene each. To evaluate the extent and the complexity of the genomic(More)
The pseudocapsule (PC) of the uterine leiomyoma (UL) is an anatomic entity that surrounds the myoma separating it from the myometrium (UM). Although a number of microarray experiments have identified differences in gene expression profile in the UL when compared with the UM, there is a lack of systematic studies on the PC. In this study, quantitative RT-PCR(More)