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Biosynthesis of astaxanthin in tobacco leaves by transplastomic engineering.
TLDR
The tobacco transformants synthesized a novel carotenoid 4-ketoantheraxanthin, which was found to be similar to that of wild-type plants under ambient CO2 concentrations of 1500 micromol photons m(-2) s(-1) light intensity.
Structure-physicochemical function relationships of soybean glycinin at subunit levels assessed by using mutant lines.
TLDR
There was no significant relationship among the other physicochemical properties of the normal glycinin (11S) or the mutant glycinins composed of only group I subunits (group I-glycinin), and this order was consistent with that of the length of their hypervariable regions.
Characterization of bacterial β-carotene 3,3′-hydroxylases, CrtZ, and P450 in astaxanthin biosynthetic pathway and adonirubin production by gene combination in Escherichia coli
Abstractβ-Carotene hydroxylase (CrtZ) is one of rate-limiting enzymes for astaxanthin production. A complementation analysis was conducted using Escherichia coli transformants to compare the
Characterization of two β-carotene ketolases, CrtO and CrtW, by complementation analysis in Escherichia coli
TLDR
The results obtained up to the present clearly suggest that the bacterial crtW and crtZ genes are a combination of the most promising gene candidates for developing recombinant hosts that produce astaxanthin as the predominant carotenoid.
Characterization of β -Carotene Ketolases, CrtW, from Marine Bacteria by Complementation Analysis in Escherichiacoli
A complementation analysis was performed in Escherichiacoli to evaluate the efficiency of β-carotene ketolases (CrtW) from the marine bacteria Brevundimonas sp. SD212, Paracoccus sp. PC1 (Alcaligenes
Identification of the Bile Acid-binding Region in the Soy Glycinin A1aB1b Subunit
TLDR
Results indicate that the 129-134 residue region (VAWWMY) with high hydrophobicity was important for bile acid-binding of A1a.
Characterization of Sphingomonas aldehyde dehydrogenase catalyzing the conversion of various aromatic aldehydes to their carboxylic acids
TLDR
Interestingly, E. coli expressing phnN was able to biotransform a variety of not only aromatic aldehydes, but also aromatic alcohols to carboxylic acids.
Component Fatty Acids of Acidic Glycerophospholipids in Rice Grains: Universal Order of Unsaturation Index in Each Lipid among Varieties
TLDR
In five acidic glycerophospholipids from grains of japonica and indica rices, phosphatidylglycerol had commonly the lowest unsaturation index and cardiolipin had the highest and the order of unasaturation index in each lipid did not depend on the variety of rice.
Characterization of four Rhodococcus alcohol dehydrogenase genes responsible for the oxidation of aromatic alcohols
TLDR
A phylogenetic analysis of the amino acid sequences of these four enzymes indicated that they differed from other Zn-dependent ADHs.
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