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Group-specific primer and probe sets to detect methanogenic communities using quantitative real-time polymerase chain reaction.
The primer and probe sets designed in this study can be used to detect and quantify the order-level methanogenic groups in anaerobic biological processes and various environments. Expand
Absolute and relative QPCR quantification of plasmid copy number in Escherichia coli.
Real-time QPCR based methods for determination of plasmid copy number in recombinant Escherichia coli cultures are presented and are convenient to perform and cost-effective compared to the traditionally used Southern blot method. Expand
A comprehensive microbial insight into two-stage anaerobic digestion of food waste-recycling wastewater.
The overall process performance was relatively stable compared to the dynamic changes in the microbial structures and the acidogenic performance in a two-stage anaerobic digestion system treating food waste-recycling wastewater. Expand
Use of real-time PCR for group-specific quantification of aceticlastic methanogens in anaerobic processes: population dynamics and community structures.
The TaqMan quantitative PCR (QPCR) method was used to detect and quantify the 16S rRNA genes of aceticlastic methanogens at different taxonomic levels, and was successful to demonstrate probe nesting at the levels of order and family. Expand
Methanogenic population dynamics assessed by real-time quantitative PCR in sludge granule in upflow anaerobic sludge blanket treating swine wastewater.
A pilot-scale upflow anaerobic sludge blanket treating swine wastewater was operated for 382 days to evaluate the process performance and methanogenic population dynamics, suggesting that hydrogenotrophic methanogenesis, with the syntrophic oxidation of volatile fatty acids (VFAs), was a major route of methane formation. Expand
Quantitative analysis of methanogenic community dynamics in three anaerobic batch digesters treating different wastewaters.
The results suggested that more attention to quantitative as well as qualitative approaches on microbial communities is needed for fundamental understanding of anaerobic processes, particularly under dynamic or transitional conditions. Expand
Real-time PCR determination of rRNA gene copy number: absolute and relative quantification assays with Escherichia coli
Real-time polymerase chain reaction (PCR)-based methodology for the determination of rRNA gene (rrn) copy number was introduced and demonstrated and was successfully applied to determine the rrn copy number in E. coli cells. Expand
Monitoring thiocyanate-degrading microbial community in relation to changes in process performance in mixed culture systems near washout.
The experimental results suggest that the structure of a microbial thiocyanate-degrading community at a given SRT would not be determined only by the washout effect, contrary to the general notion that the community structures in continuous systems will be controlled by thewashout of microbial populations. Expand
The effect of calcium on the anaerobic digestion treating swine wastewater
Abstract A series of experiments with 0, 1, 3, 5, and 7 g Ca 2+ /l using calcium chloride were performed to evaluate the effect of various calcium concentrations on anaerobic digestion of swineExpand
Selective optimization in thermophilic acidogenesis of cheese-whey wastewater to acetic and butyric acids: partial acidification and methanation.
The two-phase process clearly showed a better performance in management of cheese-whey wastewater over the single-phase anaerobic system, and also showed a higher rate in combined productions of acetic and butyric acids than that at optimum conditions forbutyric acid production. Expand