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DAPI analogs containing an imidazoline ring or a tetrahydropyrimidine ring have been synthesized to study DNA binding properties. Spectroscopic (absorption, CD, flow dichroism and fluorescence) and viscosity measurements indicate that DAPI analogs interact with DNA both by intercalation and by groove binding. The solution structures of complexes between(More)
We have examined optical properties of a fluorene derivative with two positively charged substituents, 1,1'- diethyl-4,4'-(9,9-diethyl-2,7-fluorenediyl-2,1-ethanediyl)dipyridinium perchlorate (1), in water. The photoluminescence quantum yield of 1 was relatively high (35%) for use as a fluorescent probe in water. We also examined two-photon absorption (TPA)(More)
Here we describe the design and synthesis of a bifunctional two-photon fluorescence probe, N,N'-‍dimethyl-4,4'-(biphenyl-2,1-ethenediyl)dipyridinium hexafluorophosphate (BP6). HeLa, Hek293, and Paramecium caudatum cells were stained with BP6. BP6 accumulated on the mitochondria of all three cell types when the mitochondrial membrane potential was high. As(More)
We have synthesized two novel alkyl-linked bis(benzimidazole) compounds related to pentamidine. The solution structures of 1:1 complexes of these compounds with the self-complementary DNA duplexes, d(CGCGAATTCGCG)2 (A2T2) and d(CGCAAATTTGCG)2 (A3T3) have been studied by one- and two-dimensional 1H NMR spectroscopy. The intermolecular NOE data of the A3T3(More)
We have synthesized novel alkyl-linked bis(benzimidazole) compounds and studied their DNA binding properties by spectroscopic (absorption, CD, flow dichroism and fluorescence) and viscosity measurements. The results indicate that bis(benzimidazole) compounds interact with DNA both by intercalation and by groove binding.
Solution structures of the 1:1 complexes of an alkyl-linked bis(benzimidazole) compound (4; Figure 1(a)) with the self-complementary DNA duplexes, d(CGCGAATTCGCG)2 and d(CGCAAATTTGCG)2, have been studied by 1H NMR spectroscopy and molecular mechanics calculations. The intermolecular NOE data and the energy-minimized geometries of the 1:1 complexes indicate(More)
The interaction of quinacrine (QAC) enantiomers with poly(A).poly(U) and poly(I).poly(C) has been studied by fluorescence spectroscopy. The fluorescence and fluorescence-excitation spectra of QAC enantiomers complexed with the polyribonucleotides showed a marked dependence on the excitation and emission wavelengths. This behavior of the bound QAC(More)
The effect of electric field strength of the selectivity filter on ionic selectivity of the sodium channel has been studied by ab initio molecular orbital calculations. On the basis of the previous method, two kinds of model systems, the FCO2M system and the FCO2M-H2O system, were studied. In this study, a FCO2- molecule, which is substituted for a HCO2-(More)
Visible absorption and circular dichroism (CD) spectra have been measured for complexes formed between nucleic acids (calf thymus DNA, poly(rA).poly(rU) and poly(rI).poly(rC)) and 9-aminoacridines (quinacrine, acranil and 9-amino-6-chloro-2-methoxy acridine). With poly(rA).poly(rU), a new absorption band was observed at longer wavelengths. The nucleic(More)