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Bacterial wilt-resistant plants were obtained using a tomato tissue culture system. A virulent strain ofPseudomonas solanacearum secreted some toxic substances into the culture medium. Leaf explant-derived callus tissues which were resistant to these toxic substances in the culture filtrate were selectedin vitro and regenerated into plants. These plants(More)
Cochliobolus heterostrophus Tub1 described here is the first beta-tubulin gene characterized from a naturally occurring benomyl-resistant ascomycete plant pathogen. The gene encodes a protein of 447 amino acids. The coding region of Tub1 is interrupted by three introns, of 116, 55, and 56 nt, situated after codons 4, 12, and 53, respectively. As a result of(More)
An efficient method, called the culture plate method, was devised for microinjection of foreign materials into nuclei of tomato callus cells. The culture plate method, used in this study, is advantageous because cells suitable for microinjection can be selected microscopically and the injected cells subsequently cultured in the same plate. With this(More)
Tobacco mosaic virus-resistant tobacco was selected in vitro using callus tissues induced from axillary buds of systemically infected tobacco plants. Callus lines in which the virus was continuously multiplying were first isolated and redifferentiated into shoots. By the procedure, non-diseased, healthy shoots were successfully isolated from diseased(More)
(181 LD 8.'16 Fig. 1. Paired testis weight (M+SEM) of hamsters at the age of 45 days, kept in long (LD 16:8) or short (LD 8:16) photoperiods. At the age of 6days, males were implanted with empty (Pla) or melatonin-filled (Mel) silastic tubes of different lengths (1, 2, or 3 cm), or were left untreated (Con). Open bars absolute weight, left Ordinate; hatched(More)
Resistant lines of strawberry to the fungal wilt disease caused by Fusarium oxysporum f. sp. fragariae were selected strawberry plants regenerated from leaf-derived callus tissues. Regenerants were transplanted to a field heavily infested with this pathogen, and normally growing plants were selected as the putative resistant lines. Daughter plants produced(More)
Cholesteric liquid crystals formed by esters of cholesterol and some chiral nematogenic compounds exhibit an additional helically structured liquid-crystalline phase slightly below the clearing point [1]; it is termed ~ Blue Phase" (BP) because of its striking light-reflecting properties. Recently, we reported that some optical properties, e.g., selective(More)
To clone cDNAs of mRNA specifically expressed at the infection sites, we applied the polymerase chain reaction (PCR) combined with pricking microinjection to barley coleoptile epidermis inoculated with powdery mildew pathogen. In essence, first-strand cDNAs were synthesized in situ the needle-pricked epidermal cells in which fungal haustoria had formed, and(More)