Seiji Hayashi

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A new panel of mAbs was prepared to a stromal cell line known to support lymphocytes in Whitlock-Witte type long-term bone marrow cultures. These antibodies were then screened with a cell adhesion assay and four were selected that inhibited the binding of B lineage cells to stromal cell monolayers. Immunofluorescent and biochemical analyses revealed that(More)
Tetraspanins CD9 and CD81 facilitate the fusion between gametes, myoblasts, or virus-infected cells. Here, we investigated the role of these tetraspanins in the fusion of mononuclear phagocytes. Expression of CD9 and CD81 and their complex formation with integrins were up-regulated when blood monocytes were cultured under normal conditions. Under fusogenic(More)
Cells release adenosine 5'-triphosphate (ATP) into the extracellular space in response to various stimuli. This released ATP plays an important physiological role in cell-to-cell signal transduction. The bulk ATP concentration can be detected using a conventional luciferin-luciferase assay. However, the ATP concentration in the vicinity of the cell surface(More)
The Wilms' tumor gene WT1 consists of 10 exons and encodes a zinc finger transcription factor. There are four major WT1 isoforms resulting from alternative splicing at two sites, exon 5 (17AA) and exon 9 (KTS). All major WT1 isoforms are overexpressed in leukemia and solid tumors and play oncogenic roles such as inhibition of apoptosis, and promotion of(More)
abstract To examine a possible relation between the swelling-induced ATP release pathway and the volume-sensitive Cl Ϫ channel, we measured the extracellular concentration of ATP released upon osmotic swelling and whole-cell volume-sensitive Cl Ϫ currents in a human epithelial cell line, Intestine 407, which lacks expression of cystic fibrosis transmembrane(More)
Complete mitochondrial 16S rRNA gene sequences from 35 caenogastropods were obtained to evaluate the phylogenetic relationship of the family Buccinidae at the intra-supra familial level. With respect to intergeneric relationships within the family, the molecular phylogeny supported three clades with statistical significance: Buccinum + Neptunea, Engina +(More)
To understand radiocesium transfer in the forest insect food web, we investigated the activity concentrations of radiocesium in forest insects in the Fukushima and Ibaraki Prefectures approximately 1.5-2.5 years after the Fukushima Dai-ichi Nuclear Power Plant. We analyzed 34 species of insects sampled from 4 orders and 4 feeding functional groups(More)
After the reversion of Okinawa (Ryukyu Islands) to Japan in 1972, extensive urban and agricultural development resulted in a significant increase in sediment discharge to coastal waters. The release of sediment has caused the degradation of freshwater and coastal ecosystems and biodiversity. A consideration for catchment-to-reef continua, as well as(More)
To investigate differences in culturable freshwater bacterioplankton between summer and winter, we examined specific bacterioplankton in the low temperature surface water of two shallow, hypereutrophic, temperate lakes in Japan over two winters, and compared our results with the previously published data. We used the size-exclusion assay method (SEAM), a(More)
Running title: Voltage Dependence of ion pumping by pHR. 2 SUMMARY Natronomonas pharaonis halorhodopsin (pHR) is an archaeal rhodopsin functioning as an inward-directed light-driven Cl-pump. To characterize the electrophysiological features of the Cl-pump activity of pHR, we expressed pHR in Xenopus laevis oocytes and analyzed its photo-induced Cl-pump(More)