Ad26-vector based COVID-19 vaccine encoding a prefusion stabilized SARS-CoV-2 Spike immunogen induces potent humoral and cellular immune responses
- R. Bos, L. Rutten, H. Schuitemaker
- BiologybioRxiv
- 30 July 2020
In vitro characterization demonstrated that the introduction of stabilizing substitutions increased the ratio of neutralizing versus non-neutralizing antibody binding, suggestive for a prefusion conformation of the S protein, and the wild type signal peptide was best suited for the correct cleavage needed for a natively-folded protein.
The C Terminus of SipC Binds and Bundles F-actin to Promote Salmonella Invasion
- Sebenzile K. Myeni, Daoguo Zhou
- BiologyJournal of Biological Chemistry
- 8 March 2010
It is demonstrated that amino acid residues 221–260 and 381–409 of full-length SipC were indispensable for its actin binding and bundling activities and Salmonella mutant strains lacking the actin bundling activity were less invasive into HeLa cells.
Brucella Modulates Secretory Trafficking via Multiple Type IV Secretion Effector Proteins
- Sebenzile K. Myeni, R. Child, J. Celli
- BiologyPLoS Pathogens
- 1 August 2013
It is demonstrated that Brucella modulates secretory trafficking via multiple T4SS effector proteins that likely act coordinately to promote BrucellA pathogenesis.
Insight into Bacterial Virulence Mechanisms against Host Immune Response via the Yersinia pestis-Human Protein-Protein Interaction Network
- Huiying Yang, Y. Ke, Z. Du
- BiologyInfection and Immunity
- 12 September 2011
Topological analysis of the interaction network revealed that human proteins targeted by Y. pestis were significantly enriched in the proteins that are central in the human protein-protein interaction network, which reflects common characteristics of pathogen-host interaction that bacterial pathogens have evolved to evade host innate immune response by interacting with proteins in those signaling pathways.
Capsid-like particles decorated with the SARS-CoV-2 receptor-binding domain elicit strong virus neutralization activity
- Cyrielle Fougeroux, Louise Goksøyr, A. Sander
- BiologyNature Communications
- 28 July 2020
The authors generate a capsid-like particle based vaccine candidate displaying the receptor-binding domain of the SARS-CoV-2 spike protein and show induction of neutralizing antibodies after intramuscular prime-boost immunization in mice.
Ad26 vector-based COVID-19 vaccine encoding a prefusion-stabilized SARS-CoV-2 Spike immunogen induces potent humoral and cellular immune responses.
- R. Bos, L. Rutten, H. Schuitemaker
- Biologynpj Vaccines
- 28 September 2020
In vitro characterization demonstrated that the introduction of stabilizing substitutions increased the ratio of neutralizing versus non-neutralizing antibody binding, suggestive for a prefusion conformation of the S protein, and the wild-type signal peptide was best suited for the correct cleavage needed for a natively folded protein.
SipB-SipC Complex Is Essential for Translocon Formation
- Sebenzile K. Myeni, Lu Wang, Daoguo Zhou
- BiologyPLoS ONE
- 27 March 2013
A mechanism whereby SipC binds SipB through its C-terminal region to facilitate membrane-insertion and subsequent translocon formation in the host cell membrane is proposed.
Immunogenicity and efficacy of one and two doses of Ad26.COV2.S COVID vaccine in adult and aged NHP
- L. Solforosi, H. Kuipers, R. Zahn
- Medicine, BiologyJournal of Experimental Medicine
- 28 April 2021
While single-dose COVID-19 vaccines are preferred, two-dose regimens may improve efficacy. Immunogenicity of Ad26.COV2.S is improved in NHPs after a second dose, whereas both regimens protect animals…
Ad26.COV2.S protects Syrian hamsters against G614 spike variant SARS-CoV-2 and does not enhance respiratory disease
- Joan E. M. van der Lubbe, S. R. Rosendahl Huber, F. Wegmann
- Biology, Medicinenpj Vaccines
- 19 March 2021
Preclinical data confirm efficacy of a one-dose vaccine regimen with Ad26.COV2.S in this G614 spike SARS-CoV-2 virus variant Syrian hamster model, show the added benefit of a second vaccine dose, and demonstrate that there are no signs of VAERD under conditions of suboptimal immunity.
Two-Component Nanoparticle Vaccine Displaying Glycosylated Spike S1 Domain Induces Neutralizing Antibody Response against SARS-CoV-2 Variants
- Linda van Oosten, Jort J. Altenburg, G. Pijlman
- BiologymBio
- 12 October 2021
The versatile and scalable baculovirus expression vector system is used to generate a two-component nanoparticle vaccine to induce potent neutralizing antibody responses against SARS-CoV-2 variants.
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